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    Human Epidermal Keratinocytes: HEK 人表皮角質形成細胞：HEK

    產品型號： 102K-05a

    HEKHuman Epidermal Keratinocytes (HEK) provide an excellent model to study epithelial function and disease, skin biology, toxicology and HPV infection. When grown on inserts and provided a liquid/air interface, they differentiate into a stratified squamous epithelium, forming a physiological 3D tissue. HEK comprise 90 % of all cells in the epidermis, the outermost skin layer.  They produce a barrier, the intermediate filament keratin.  Keratinocytes obtain melanin from melanocytes (HEM) and store it as protection from UV radiation.  We highly recommend optimized Human EpiVita Media to enhance the performance and in vitro lifespan of HEK.
    Click thumbnails for more videos of HEK in action:
    HEK from Cell Applications, Inc. have been utilized in numerous research publications, for example in basic research to investigate mechanisms of cell senescence and develop ways to extend cell life span.  Others have used HEK to design a method to isolate proteins for use in basic researh, and show higher relevance of primary keratinocytes when compared to key differences exhibited by immortalized keratinocyte cell lines.  Pharmaceutical companies employ HEK to provide in vitro human skin models that assess effects of cosmetic formulations or anti-inflammatory properties of proposed anti-psoriasis drugs.  Translational research on keratinocytes benefits from their ability to model the wound healing response, or help develop serum tests for patients with blistering diseases.  HEK are also utilized to understand aggressiveness of cancer phenotypes, investigate compounds for potential topical melanoma treatments, and even develop siRNA therapy targeting oncogenes.  In addition, HEK help unravel the oncogenic potential of HPV and test approaches for treating HPV lesions.
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    DETAILS

    Tissue
    Normal healthy human foreskin or adult skin
    QC
    No bacteria, yeast, fungi, mycoplasma, virus
    Bioassay
    Attach, spread, proliferate in Growth Med
    Cryovial
    500,000 HEK (Primary culture) frozen in Basal Medium w/ 10% FBS, 10% DMSO
    Kit
    Cryovial HEK (102-05), Epi-Vita Growth Medium (141-500a for adult; 141-500 for fetal/neonatal), Subcltr Rgnt Kit (090K)
    Proliferating
    In Serum-Fr Gr Med, psg 1, flasks or plates
    Doublings
    At least 16 in keratinocyte media
    Applications
    Laboratory research use only (RUO). Not for human, clinical, diagnostic or veterinary use.

     

    PRODUCTS

    PRODUCT SIZE CAT.#
    Cryopreserved HEK, Adult: 5x10^5 Cells (Adult), Medium & Subculture Reagents (See Details tab for specifics) 1 Kit 102K-05a
    Cryopreserved HEK, Adult: Frozen HEK (5x10^5) 1 Cryovial 102-05a
    Cryopreserved HEK, Fetal: 5x10^5 Cells (Fetal), Medium & Subculture Reagents (See Details tab for specifics) 1 Kit 102K-05f
    Cryopreserved HEK, Fetal: Frozen HEK (5x10^5) 1 Cryovial 102-05f
    Cryopreserved HEK, Neonatal: 5x10^5 Cells (Neonatal), Medium & Subculture Reagents (See Details tab for specifics) 1 Kit 102K-05n
    Cryopreserved HEK, Neonatal: Frozen HEK (5x10^5) 1 Cryovial 102-05n

     

    RELATED PRODUCTS

    PRODUCT SIZE CAT.#
    Human EpiVita Growth Medium for Adult Cells: All-in-one ready-to-use, standard Epithelial Cell Medium, for adult cells. Does not contain FBS. 500 ml 141-500a
    Human EpiVita Growth Medium Kit for Adult Cells: Basal medium & growth supplement sold together packaged separately, for adult cells. Does not contain FBS. Yields 500 ml 141K-500a
    Human EpiVita Growth Supplement for Adult Cells: Added to Basal Medium to create Growth Medium, for adult cells. Does not contain FBS. 5 ml 141-GSa
    Human EpiVita Growth Medium for Neonatal Cells: All-in-one ready-to-use, standard Epithelial Cell Medium, for neonatal and fetal cells. Does not contain FBS. 500 141-500
    Human EpiVita Growth Medium Kit for Neonatal Cells: Basal medium & growth supplement sold together packaged separately, for neonatal and fetal cells. Does not contain FBS. Yields 500 ml 141K-500
    Human EpiVita Growth Supplement for Neonatal Cells: Added to Basal Medium to create Growth Medium, for neonatal and fetal cells. Does not contain FBS. 5 ml 141-GS
    Human EpiVita Basal Medium: Basal medium (contains no growth supplement).  Add GS before use. 500 ml 140-500

     

    EXTENDED FAMILY PRODUCTS

    PRODUCT SIZE CAT.#
    Human EpiVita Defined Growth Medium: All-in-one ready-to-use 500 141DGM-500
    Human EpiVita Defined Growth Medium Kit: Basal medium & growth supplement sold together packaged separately Yields 500 ml 141DGMK-500
    Human EpiVita Basal Medium: Basal medium (contains no growth supplement).  Add GS before use. 500 ml 140-500
    Human EpiVita Defined Growth Supplement: Added to Basal Medium to create Growth Medium 5 ml 141DGM-GS
    Keratinocyte Calcium-Free Growth Medium: Growth medium without calcium 500 ml 133-500
    Keratinocyte Calcium-Free Growth Medium Kit, for fetal and neonatal cells: Growth medium kit without calcium Yields 500 ml 133K-500
    Keratinocyte Calcium-Free Growth Medium Kit, for adult cells: Growth medium kit for adult cells without calcium Yields 500 ml 133K-500a
    Keratinocyte Calcium-Free Basal Medium: Basal medium without calcium 500 ml 132-500
    Keratinocyte Calcium-Free Growth Supplement: Growth supplement without calcium 5 ml (3 parts) 133-GS
    3-D Skin Model: HEK differentiated into stratified squamous epithelium on PCF inserts. Available only in the US. 12 Inserts 102-3D-12
    3-D Skin Model: HEK differentiated into stratified squamous epithelium on PCF inserts. Available only in the US. 24 Inserts 102-3D-24
    3-D Skin Model: Cells, Media, Reagents & Inserts (See Details tab for specifics) 1 Kit 102K-3D
    Polyclonal CXCR1 Rabbit Antibody: Polyclonal CXCR1 Rabbit Antibody 100 ul CA0755
    Polyclonal CXCR2 Rabbit Antibody: Polyclonal CXCR2 Rabbit Antibody 100 ul CA0757
    Human Epidermal Growth Factor (EGF): Human Epidermal Growth Factor 100 ug RP1026-100
    Human Epidermal Growth Factor (EGF): Human Epidermal Growth Factor 500 ug RP1026-500
    Human Epidermal Growth Factor (EGF): Human Epidermal Growth Factor 1000 ug RP1026-1000
    HEK RNA: Total RNA prepared from Human Epidermal Keratinocytes, adult 10 ug 102-R10a
    HEK RNA: Total RNA prepared from Human Epidermal Keratinocytes, adult 25 ug 102-R25a
    HEK RNA: Total RNA prepared from Human Epidermal Keratinocytes, fetal 10 ug 102-R10f
    HEK RNA: Total RNA prepared from Human Epidermal Keratinocytes, fetal 25 ug 102-R25f
    HEK RNA: Total RNA prepared from Human Epidermal Keratinocytes, neonatal 10 ug 102-R10n
    HEK RNA, Neonatal: Total RNA prepared from Human Epidermal Keratinocytes, neonatal 25 ug 102-R25n
    Subculture Reagent Kit: 100 ml each of HBSS, Trypsin/EDTA & Trypsin Neutralizing Solution 1 Kit 090K
    Human EGF, Animal-Free: Human Epidermal Growth Factor, Animal-Free 100 ug RP1026AF-100
    Human EGF, Animal-Free: Human Epidermal Growth Factor, Animal-Free 500 ug RP1026AF-500
    Human EGF, Animal-Free: Human Epidermal Growth Factor, Animal-Free 1000 ug RP1026AF-1000

    General Instructions for Culturing Human Epidermal Keratinocytes (HEK)

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