Human Skeletal Muscle Cells: HSkMC 人體骨骼肌細胞：HSkMC
產品型號： 150K-05f
Human Skeletal Muscle Cells (HSkMC), isolated from adult or fetal donor limbs are positive for sarcomere myosin and can undergo differentiation to exhibit actin and myosin myofilaments. Our HSkMC provide a useful model system to study many aspects of muscular function and disease. Skeletal Muscle Cells play an instrumental role in the glucose metabolism and diabetes, so select lots undergo an additional set of Pre-Screening at Cell Applications, Inc. to demonstrate AMPK signaling and responsiveness to insulin stimulation.
HSkMC from Cell Applications, Inc. have been used to:
- Serve as a differentiated control in a study of developmental regulator genes in hESC
- Characterize statin-induced gene expression changes and demonstrate cytotoxic effect of statins in skeletal muscle cells
- Identify molecular mechanisms of mitochondrial myopathy and sideroblastic anemia resulting from a missense mutation in the PUS1 gene
- Investigate chemokine-like factor expression in the idiopathic inflammatory myopathies
- Demonstrate that fasting activates AceCS2 gene expression by inducing KLF15 transcription factor
- Characterize human FGFR3-positive sarcoma-initiating stem cells
- Identify trans-Golgi network proteins and Notch and Hedgehog pathways as putative targets for rhabdomyosarcoma therapy
- Show that riluzole muscle relaxant effects are mediated by inhibition of INa and stimulation of BKC-channel activity
- Develop nitric esters that combine the pharmacological functions of NO and muscle relaxation properties for treatment of muscular diseases
- Develop biodegradable polymer-based transgene delivery vectors for muscular dystrophy treatment
- Design optimal coating for orthopedic metallic implants
DETAILS
|
Tissue
|
Normal healthy human limb skeletal muscle
|
|
QC
|
No bacteria, yeast, fungi, mycoplasma, virus
|
|
Character
|
Multinucleated myotubes form after culture in fusion medium
|
|
Bioassay
|
Attach, spread, proliferate in Growth Med
|
|
Cryovial
|
500,000 HSkMC (2nd passage) frozen in Basal Medium w/ 10% FBS, 10% DMSO
|
|
Kit
|
Cryovial frozen HSkMC (150-05), Growth Medium (151-500), Subcltr Rgnt Kit (090K)
|
|
Proliferating
|
Shipped in Tsfr Med, psg 3, flasks or plates
|
|
Doublings
|
At least 15
|
|
Applications
|
Laboratory research use only (RUO). Not for human, clinical, diagnostic or veterinary use.
|
PRODUCTS
| PRODUCT | SIZE | CAT.# |
| Cryopreserved HSkMC, Fetal: 5x10^5 Cells (Fetal), Medium & Subculture Reagents (See Details tab for specifics) | 1 Kit | 150K-05f |
| Cryopreserved HSkMC, Adult: 5x10^5 Cells (Adult), Medium & Subculture Reagents (See Details tab for specifics) | 1 Kit | 150K-05a |
| Cryopreserved HSkMC, Pre-Screened, Fetal: 5x10^5 Cells (Pre-Screened, Fetal), Medium & Subculture Reagents (See Details tab for specifics) | 1 Kit | S150K-05f |
| Cryopreserved HSkMC, Adult: Frozen HSkMC (5x10^5) | 1 Cryovial | 150-05a |
| Cryopreserved HSkMC, Fetal: Frozen HSkMC (5x10^5) | 1 Cryovial | 150-05f |
| Cryopreserved HSkMC, Pre-Screened, Fetal: Frozen HSkMC (5x10^5) | 1 Cryovial | S150-05f |
| Cryopreserved HSkMC-COPD, Pre-Scr, Adult: 5x10^5 Cells (from donor with COPD, Pre-Screened, Adult), Medium & Subculture Reagents (See Details tab for specifics) | 1 Kit | S150COPDK-05a |
| Cryopreserved HSkMC-COPD, Pre-Scr, Adult: Frozen HSkMC-COPD from donor with Chronic Obstructive Pulmonary Disease, Adult (5x10^5) | 1 Cryovial | S150COPD-05a |
| Cryopreserved HSkMC-T2D, Adult: 5x10^5 Cells (from donor with Type 2 Diabetes, Adult), Medium & Subculture Reagents (See Details tab for specifics) | 1 Kit | 150T2DK-05a |
| Cryopreserved HSkMC-T2D, Adult: Frozen HSkMC from donor with Type 2 Diabetes (5x10^5) | 1 Cryovial | 150T2D-05a |
| Proliferating HSkMC, Adult: Actively growing, dividing cells in medium | T-25 Flask | 151-25a |
| Proliferating HSkMC, Adult: Actively growing, dividing cells in medium | T-75 Flask | 151-75a |
| Proliferating HSkMC, Fetal: Actively growing, dividing cells in medium | T-25 Flask | 151-25f |
| Proliferating HSkMC, Fetal: Actively growing, dividing cells in medium | T-75 Flask | 151-75f |
| Proliferating HSkMC, Pre-Screened, Fetal: Actively growing, dividing cells in medium | T-25 Flask | S151-25f |
| Proliferating HSkMC, Pre-Screened, Fetal: Actively growing, dividing cells in medium | T-75 Flask | S151-75f |
RELATED PRODUCTS
| PRODUCT | SIZE | CAT.# |
| HSkMC Growth Medium: All-in-one ready-to-use | 500 ml | 151-500 |
| HSkMC Growth Medium Kit: Basal medium & growth supplement sold together packaged separately | Yields 500 ml | 151K-500 |
| HSkMC Basal Medium: Basal medium (contains no growth supplement). Add GS before use. | 500 ml | 150-500 |
| HSkMC Growth Supplement: Added to Basal Medium to create Growth Medium | 50 ml (2 parts) | 151-GS |
| HSkMC Differentiation Medium: Promotes cells to change from one type to another, more specialized | 250 ml | 151D-250 |
EXTENDED FAMILY PRODUCTS
| PRODUCT | SIZE | CAT.# |
| Mouse Interleukin-6 Antibody: Mouse Interleukin-6 Antibody | 100 ul | CP10325 |
| Freezing Medium: For general cryopreservation of most primary cells. Contains FBS & DMSO. | 50 ml | 040-50 |
| Cyto-X Cell Counting Reagent: 500 tests | 1 Bottle | 028-01 |
| Cyto-X Cell Counting Reagent Sample: 100 tests | Sample | 028-S |
| HSkMC RNA, Adult: Total RNA prepared from Human Skeletal Muscle Cells, adult | 10 ug | 150-R10a |
| HSkMC RNA, Adult: Total RNA prepared from Human Skeletal Muscle Cells, adult | 25 ug | 150-R25a |
| HSkMC RNA, Fetal: Total RNA prepared from Human Skeletal Muscle Cells, fetal | 10 ug | 150-R10f |
| HSkMC RNA, Fetal: Total RNA prepared from Human Skeletal Muscle Cells, fetal | 25 ug | 150-R25f |
| HSkMC, Multinucleated Myotubes RNA, Fetal: Total RNA prepared from Human Skeletal Muscle Cells, Differentiated, fetal | 10 ug | 150D-R10f |
| HSkMC, Multinucleated Myotubes RNA, Fetal: Total RNA prepared from Human Skeletal Muscle Cells, Differentiated, fetal | 25 ug | 150D-R25f |
| Human Interleukin-6 (IL-6): Human Interleukin-6 | 20 ug | RP1010-20 |
| Human Interleukin-6 (IL-6): Human Interleukin-6 | 100 ug | RP1010-100 |
| Human Interleukin-6 (IL-6): Human Interleukin-6 | 1000 ug | RP1010-1000 |
| Human Skeletal Muscle RNA: Total RNA prepared from human skeletal muscle tissue | 50 ug | 1H60-50 |
| Human Skeletal Muscle RNA: Total RNA prepared from human skeletal muscle tissue | 250 ug | 1H60-250 |
| Subculture Reagent Kit: 100 ml each of HBSS, Trypsin/EDTA & Trypsin Neutralizing Solution | 1 Kit | 090K |
| Mouse Myostatin Antibody: Mouse Myostatin Antibody | 100 ul | CP10315 |
| Human Myostatin (GDF-8): Human Myostatin | 10 ug | RP1022-10 |
| Human Myostatin (GDF-8): Human Myostatin | 100 ug | RP1022-100 |
| Human Myostatin (GDF-8): Human Myostatin | 1000 ug | RP1022-1000 |
| Human Myostatin Propeptide: Human Myostatin Propeptide | 25 ug | RP1142-25 |
| Human Myostatin Propeptide: Human Myostatin Propeptide | 100 ug | RP1142-100 |
| Human Myostatin Propeptide: Human Myostatin Propeptide | 1000 ug | RP1142-1000 |
| Human IL-6, Animal Free: Human Interleukin-6, Animal-Free | 20 ug | RP1010AF-20 |
| Human IL-6, Animal Free: Human Interleukin-6, Animal-Free | 100 ug | RP1010AF-100 |
| Human IL-6, Animal Free: Human Interleukin-6, Animal-Free | 1000 ug | RP1010AF-1000 |
| Skeletal Muscle Cell Starvation Medium: Use when cells need to be starved overnight to 24 hrs before experiment | 250 ml | 151S-250 |
Inline content