Human Pulmonary Artery Endothelial Cells: HPAEC 人肺動脈內皮細胞：HPAEC
產品型號： 302K-05a
Human Pulmonary Artery Endothelial Cells (HPAEC) provide an excellent model system to study many aspects of cardiovascular function and disease, including diabetes-associated complications, mechanisms of endothelial dysfunction caused by environment, oxidative stress, hypoxia and inflammation, as well as mode of action of various natural compounds and drug candidates.
Note that several publications describe the differences between endothelial cells from different vascular beds and between macro- and micro-vascular cells from the same organ, highlighting the importance of confirming any new findings on cell lots obtained from several different origins. Cell Applications, Inc. offers the widest variety of endothelial cells, and is well equipped to meet this need.
HPAEC from Cell Applications have been used to investigate or demonstrate
- Vasculoprotective effects of propofol, demonstrating its potential for treating pulmonary arterial hypertension
- Hits from a high-throughput screening assay and discover anziaic acid inhibits bacterial activity
- Endothelin-1 is released by endothelial cells during acute crisis in sickle cell anemia, increases vascular constriction
- Pollutant particles cause vascular dysfunction by up-regulating clotting-related genes
- Central role of AMP-activated protein kinase in normal endothelial barrier function can be disrupted, causing endothelial hyperpermeability and lung injury
- Anti-inflammatory and cardioprotective effects of resveratrol
- cGMP prevents oxidant-induced damage to the endothelial barrier function
- Radiation causes endothelial cell senescence due to up-regulation of proliferative signaling in the presence of cell cycle arrest
- Vanadium exposure causes pulmonary vasoconstriction mediated in part by the inhibition of endothelial NO production
- Cytotoxic effects of a novel pore-forming protein, proposed as an anti-tumor agent
- Effects of Bone Morphogenic Protein-4, helping to explain why its upregulation leads to atherosclerosis and hypertension
- Macro-vascular pulmonary endothelial cells accumulate HIF-1 under hypoxic conditions
- Transportation of bioactive lipids and its silencing by siRNA
DETAILS
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Tissue
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Normal healthy human pulmonary artery
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QC
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No bacteria, yeast, fungi, mycoplasma, virus
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Character
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VIII-related Ag expression, DiI-Ac-LDL uptake
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Bioassay
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Attach, spread, proliferate in Growth Med
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Cryovial
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500,000 HPAEC (2nd passage) frozen in Basal Medium w/ 10% FBS, 10% DMSO
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Kit
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Cryovial frozen HPAEC (302-05a), Growth Medium (211-500), Subculture Rgnt Kit (090K)
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Proliferating
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Shipped in Gr Med, 3rd psg (flasks or plates)
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Doublings
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At least 15 |
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Applications
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Laboratory research use only (RUO). Not for human, clinical, diagnostic or veterinary use. |
PRODUCTS
| PRODUCT | SIZE | CAT.# |
| Cryopreserved HPAEC, Adult: 5x10^5 Cells (Adult), Medium & Subculture Reagents (See Details tab for specifics) | 1 Kit | 302K-05a |
| Cryopreserved HPAEC, Adult: Frozen HPAEC (5x10^5) | 1 Cryovial | 302-05a |
| Cryopreserved HPAEC-AS, Pre-Scr, Adult: Frozen HPAEC-AS from donor with Asthma (5x10^5) | 1 Cryovial | S302AS-05a |
| Cryopreserved HPAEC-AS, Pre-Scr, Adult: 5x10^5 Cells (from donor with Asthma, Pre-Screened, Adult), Medium & Subculture Reagents (See Details tab for specifics) | 1 Kit | S302ASK-05a |
RELATED PRODUCTS
| PRODUCT | SIZE | CAT.# |
| Human EC Basal Medium: Basal medium (contains no growth supplement). Add GS before use. | 500 ml | 210-500 |
| Human EC Basal Medium wo Phenol Red: Basal medium without growth supplement and phenol red | 500 ml | 210PR-500 |
| Human EC Serum-Free Defined Medium: Defined medium without serum designed to maintain endothelial cell monlayers in confluent cultures. Not for cell growth. | 500 ml | 113-500 |
| Human EC Serum-Free Defined Medium wo Phenol Red: Defined medium, without serum or phenol red, designed to maintain endothelial cell monlayers in 100% confluent culture. Not intended for cell growth. | 500 ml | 113PR-500 |
| Human EC Growth Medium: All-in-one ready-to-use. Optimized and best-suited for angiogenesis and other physiological studies. For accelerated growth, use Cat# 213-500. | 500 ml | 211-500 |
| Human EC Growth Medium wo Phenol Red: Growth medium without phenol red. Optimized and best-suited for angiogenesis and other physiological studies. For accelerated growth, use Cat# 213-500. | 500 ml | 211PR-500 |
| Human EC Growth Medium wo FBS: Growth medium without FBS. The complete Growth Medium is optimized and best-suited for angiogenesis and other physiological studies. For accelerated growth, use Cat# 213-500. | 500 ml | 211F-500 |
| Human EC Growth Medium wo Antibiotics: Growth medium without antibiotics. Optimized and best-suited for angiogenesis and other physiological studies. Suitable for Cytofect Transfection Kit. For accelerated growth, use Cat# 213-500. | 500 ml | 211A-500 |
| Human EC Growth Medium wo Hydrocortisone: Growth medium without hydrocortisone. The complete Growth Medium is optimized and best-suited for angiogenesis and other physiological studies. For accelerated growth, use Cat# 213-500. | 500 ml | 211H-500 |
| Human EC Growth Medium Kit: Basal medium & growth supplement sold together packaged separately. Optimized and best-suited for angiogenesis and other physiological studies. For accelerated growth, use Cat# 213-500. | Yields 500 ml | 211K-500 |
| Human EC Growth Supplement: Added to Basal Medium to create Growth Medium. The complete Growth Medium (equivalent to Cat# 211-500) is optimized and best-suited for angiogenesis and other physiological studies. For accelerated growth, use Cat# 213-500. | 15 ml | 211-GS |
| Human EC Growth Supplement wo FBS: Growth supplement without FBS | 5 ml | 211F-GS |
| Human EC Growth Supplement wo Antibiotics: Growth supplement without antibiotics | 15 ml | 211A-GS |
| Human EC Growth Supplement wo Hydrocortisone: Growth supplement without hydrocortisone | 15 ml | 211H-GS |
| Endothelial Cell Starvation Medium: Use when cells need to be starved overnight to 24 hrs before experiment | 250 ml | 209-250 |
| Endothelial Cell Starvation Medium wo Phenol Red: Starvation medium without phenol red | 250 ml | 209PR-250 |
| Human EC Growth Medium V2: All-in-one ready-to-use. Enriched with more growth factors for accelerated cell growth. For angiogenesis studies, use Cat# 211-500. | 500 ml | 213-500 |
EXTENDED FAMILY PRODUCTS
| PRODUCT | SIZE | CAT.# |
| Anti-ICAM-1: Rabbit Intercellular Adhesion Molecule-1 Antibody | 100 ul | CG1238 |
| Polyclonal Vascular Endothelial Growth Factor Antibody: Polyclonal Vascular Endothelial Growth Factor Antibody | 100 ul | CA1080 |
| Polyclonal Vascular Endothelial Growth Factor-C Antibody: Polyclonal Vascular Endothelial Growth Factor-C Antibody | 100 ul | CB3778 |
| Polyclonal VEGF Receptor 1 Antibody: Polyclonal VEGF Receptor 1 Antibody | 100 ul | CB3839 |
| Cyto-X Cell Counting Reagent: 500 tests | 1 Bottle | 028-01 |
| Cyto-X Cell Counting Reagent Sample: 100 tests | Sample | 028-S |
| Cytofect™ Endothelial Cell Transfection Kit: 250 x 24-Well Rxns | 1 Kit | TF101K |
| Cytofect™ Endothelial Cell Transfection Kit: 25 x 24-Well Rxns | 1 Sample Kit | TF101KS |
| Freezing Medium: For general cryopreservation of most primary cells. Contains FBS & DMSO. | 50 ml | 040-50 |
| Human Heart RNA: Total RNA prepared from human heart tissue | 50 ug | 1H30-50 |
| Human Heart RNA: Total RNA prepared from human heart tissue | 250 ug | 1H30-250 |
| HPAEC RNA, Adult: Total RNA prepared from Human Pulmonary Artery Endothelial Cells, adult | 10ug | 302-R10a |
| HPAEC RNA, Adult: Total RNA prepared from Human Pulmonary Artery Endothelial Cells, adult | 25ug | 302-R25a |
| Human Gamma-Interferon Inducible Protein 10 (IP-10 / CXCL10): Human gamma-Interferon Inducible Protein 10 | 25 ug | RP1127-25 |
| Human Gamma-Interferon Inducible Protein 10 (IP-10 / CXCL10): Human gamma-Interferon Inducible Protein 10 | 100 ug | RP1127-100 |
| Human Gamma-Interferon Inducible Protein 10 (IP-10 / CXCL10): Human gamma-Interferon Inducible Protein 10 | 1000 ug | RP1127-1000 |
| Subculture Reagent Kit: 100 ml each of HBSS, Trypsin/EDTA & Trypsin Neutralizing Solution | 1 Kit | 090K |
| Human Vascular Endothelial Growth Factor-121 (VEGF-121): Human Vascular Endothelial Growth Factor-121 | 10 ug | RP1116-10 |
| Human Vascular Endothelial Growth Factor-121 (VEGF-121): Human Vascular Endothelial Growth Factor-121 | 100 ug | RP1116-100 |
| Human Vascular Endothelial Growth Factor-121 (VEGF-121): Human Vascular Endothelial Growth Factor-121 | 1000 ug | RP1116-1000 |
| Human VEGF-121, Animal-Free: Human Vascular Endothelial Growth Factor-121, Animal-Free | 10 ug | RP1116AF-10 |
| Human VEGF-121, Animal-Free: Human Vascular Endothelial Growth Factor-121, Animal-Free | 100 ug | RP1116AF-100 |
| Human VEGF-121, Animal-Free: Human Vascular Endothelial Growth Factor-121, Animal-Free | 1000 ug | RP1116AF-1000 |
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