Human Chondrocytes: Re-Differentiated: HC-RD 人類軟骨細胞：重新分化：HC-RD
產品型號： 403RD-25a_403RD-25f
Human Chondrocytes Re-Differentiated (HC-RD) have been encapsulated in an alginate bead suspension. HC-RD exhibit spherical morphology and have regained their ability to produce the phenotypic markers Aggrecan and Type II Collagen. RD-HC can be used in tissue engineering experiments aimed to repair cartilage defects and to study chondrocyte differentiation and the effects of Growth factors & Cytokines on the suppression and maintenance of differentiated cartilage phenotype.
Instructions to release HC-RD from Alginate Beads
- Remove Chondrocyte Differentiation Medium
- Wash the beads 2 times with 0.155 M NaCl
- Add 3 volume of Depolymerization Solution per volume of packed alginate beads.
- Mix at room temperature for 30 minutes with gentle agitation until the beads have completely dissolved
- Pellet re-differentiated chondrocytes at 1000 rpm for 10 minutes
- Wash the cells 2 times with 0.155 M NaCl
Media Salts & Chemicals used to Release HC-RD from alginate beads:
- Calcium Chloride Solution: Formulated to induce polymerization of Sodium Alginate.
- Sodium Alginate: Encapsulates chondrocytes to maintain their phenotypic characterization after isolation or to induce cultured chondrocytes to re-differentiate to chondrocytes
- Depolymerization Solution (DS): Consists of sodium citrate, and dissolves alginate beads to release re-differentiated chondrocytes.
Details
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Tissue
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Normal healthy human articular cartilage
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QC
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No bacteria, yeast, fungi, mycoplasma, virus
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Cryovial
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N/A
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Kit
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N/A
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Proliferating
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Shipped in Differentiation Medium (flasks)
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Doublings
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N/A
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Note
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Cells shipped 10 days after encapsulation. Re-Differentiated Chondrocytes expected 3 weeks after encapsulation, retain phenotype at least 1 week after complete re-differentiation.
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Applications
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Laboratory research use only (RUO). Not for human, clinical, diagnostic or veterinary use.
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Products
| PRODUCT | SIZE | CAT.# |
| Proliferating Chondrocytes, Re-Differentiated (HC-RD), adult: Actively growing, dividing cells, in medium | T-25 Flask | 403RD-25a |
| Proliferating Chondrocytes, Re-Differentiated (HC-RD), adult: Actively growing, dividing cells, in medium | T-75 Flask | 403RD-75a |
| Proliferating Chondrocytes, Re-Differentiated (HC-RD), fetal: Actively growing, dividing cells, in medium | T-25 Flask | 403RD-25f |
| Proliferating Chondrocytes, Re-Differentiated (HC-RD), fetal: Actively growing, dividing cells, in medium | T-75 Flask | 403RD-75f |
Related Products
| PRODUCT | SIZE | CAT.# |
| HC Basal Medium: Basal medium (contains no growth supplement). Add GS before use. | 500 ml | 410-500 |
| HC Differentiation Medium: Promotes cells to change from one type to another, more specialized | 250 ml | 411D-250 |
| HC Growth Medium: All-in-one ready-to-use | 500 ml | 411-500 |
| HC Growth Medium wo Phenol Red: Growth medium without phenol red | 500 ml | 411PR-500 |
| HC Growth Medium Kit: Basal medium & growth supplement sold together packaged separately | Yields 500 ml | 411K-500 |
| HC Growth Supplement: Added to Basal Medium to create Growth Medium | 50 ml | 411-GS |
| HC Differentiation Medium wo Phenol Red: Promotes cells to change from one type to another, more specialized, without Phenol Red |
250 ml | 411DPR-250 |
Extended Family Products
| PRODUCT | SIZE | CAT.# |
| Mouse MMP-1 Antibody: Mouse MMP-1 Antibody | 100 ul | CP10422 |
| Freezing Medium: For general cryopreservation of most primary cells. Contains FBS & DMSO. | 50 ml | 040-50 |
| Chondrogenesis Kit: Used to re-differentiate chondrocytes, that have been grown in monolayers, by encapsulation in alginate beads. | 1 Kit | 072K |
| Human Connective Tissue Growth Factor (CTGF): Human Connective Tissue Growth Factor | 20 ug | RP1060-20 |
| Human Connective Tissue Growth Factor (CTGF): Human Connective Tissue Growth Factor | 100 ug | RP1060-100 |
| Human Connective Tissue Growth Factor (CTGF): Human Connective Tissue Growth Factor | 1000 ug | RP1060-1000 |
| Cyto-X Cell Counting Reagent: 500 tests | 1 Bottle | 028-01 |
| Cyto-X Cell Counting Reagent Sample: 100 tests | Sample | 028-S |
| Cytofect Chondrocyte Transfection Kit (175 x 24-Wells): 175 x 24-Well Rxns | 1 Kit | TF402K |
| Cytofect Chondrocyte Transfection Sample Kit (25 x 24-Wells): 25 x 24-Well Rxn | 1 Sample Kit | TF402KS |
| Chondrocyte RNA (HC RNA), Re-Differentiated, Adult: Total RNA prepared from Human Chondrocytes, Re-Differentiated, adult | 10 ul | 402RD-R10a |
| Chondrocyte RNA (HC RNA), Re-Differentiated, Adult: Total RNA prepared from Human Chondrocytes, Re-Differentiated, adult | 25 ul | 402RD-R25a |
| Chondrocyte RNA (HC RNA), Re-Differentiated, Fetal: Total RNA prepared from Human Chondrocytes, Re-Differentiated, fetal | 10 ul | 402RD-R10f |
| Chondrocyte RNA (HC RNA), Re-Differentiated, Fetal: Total RNA prepared from Human Chondrocytes, Re-Differentiated, fetal | 25 ul | 402RD-R25f |
| Human MMP-1 ELISA Kit: Human Matrix Metalloproteinase-1 ELISA Kit | 96 Wells | CL0458 |
| Subculture Reagent Kit: 100 ml each of HBSS, Trypsin/EDTA & Trypsin Neutralizing Solution | 1 Kit | 090K |
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