Human Fibroblast-Like Synoviocytes: Osteoarthritis: HFLS-OA 人纖維母細胞樣滑膜細胞：骨關節炎：HFLS-OA
產品型號： 408OAK-05a
Human Fibroblast-Like Synoviocytes: Osteoarthritis (HFLS-OA) provide an excellent cellular model for studying synoviocyte physiology in relation to development and treatment of osteoarthritis.
HFLS-OA from Cell Applications, Inc. have been shown to:
- Possess osteogenic, chondrogenic and adipogenic potentials
- Express about half of the mesenchymal stem cell related transcription factors and molecular markers identified in a comprehensive study
- Involve LPA–EDG2 signaling in the pathogenesis of osteoarthritis via catabolic processes
- Employ parvorvirus B19 capsid proteins to activate synoviocyte migration and induction of the inflammatory response leading to acute symmetrical polyarthropathy
Finally, all three types of HFLS (normal, RA and OA), all from Cell Applications, Inc. were used to investigate the role of human endogenous retroviruses (HERVs) in development of rheumatoid arthritis, and suggest that activated expression of different forms of HERV contribute to development of rheumatoid arthritis symptoms by different mechanisms.
DETAILS
| Tissue | Human synovial tissue from donor with osteoarthrits |
| QC | No bacteria, yeast, fungi, mycoplasma, virus |
| Cryovial | 500,000 HFLS-OA (2nd passage) frozen in Basal Medium w/ 10% FBS, 10% DMSO |
| Kit | Croyvial frozen HFLS-OA (408OA-05a), Gr Med (415-500), Subcltr Rgnt Kit (090K) |
| Proliferating | Shipped in Gr Med, 3rd psg (flasks or plates) |
| Doublings | At least 5 |
| Applications | Laboratory research use only (RUO). Not for human, clinical, diagnostic or veterinary use. |
PRODUCTS
|
PRODUCT |
SIZE | CAT.# |
| Cryopreserved HFLS-OA, Adult: 5x10^5 Cells (from donor with Osteoarthritis, Adult), Medium & Subculture Reagents (See Details tab for specifics) | 1 Kit | 408OAK-05a |
| Cryopreserved HFLS-OA, Adult: Frozen HFLS from donor with Osteoarthritis (5x10^5) | 1 Cryovial | 408OA-05a |
| Proliferating HFLS-OA, Adult: Actively growing, dividing cells in medium, from donor with Osteoarthritis | T-25 Flask | 409OA-25a |
| Proliferating HFLS-OA, Adult: Actively growing, dividing cells in medium, from donor with Osteoarthritis | T-75 Flask |
409OA-75a |
RELATED PRODUCTS
| PRODUCT | SIZE | CAT.# |
| HFLS Basal Medium: Basal medium (contains no growth supplement). Add GS before use. | 500 ml | 414-500 |
| HFLS Growth Medium: All-in-one ready-to-use | 500 ml | 415-500 |
| HFLS Growth Medium Kit: Basal medium & growth supplement sold together packaged separately | Yields 500 ml | 415K-500 |
| HFLS Growth Supplement: Added to Basal Medium to create Growth Medium | 50 ml | 415-GS |
EXTENDED FAMILY PRODUCTS
| PRODUCT | SIZE | CAT.# |
| HFLS-OA RNA, Adult: Total RNA prepared from Human Fibroblast-Like Synoviocytes-Osteoarthritis, adult | 10 ug | 408OA-R10a |
| HFLS-OA RNA, Adult: Total RNA prepared from Human Fibroblast-Like Synoviocytes-Osteoarthritis, adult | 25 ug | 408OA-R25a |
| Subculture Reagent Kit: 100 ml each of HBSS, Trypsin/EDTA & Trypsin Neutralizing Solution | 1 Kit | 090K |
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