Human Lung Microvascular Endothelial Cells: HLMVEC 人肺微血管內皮細胞：HLMVEC
產品型號： 540K-05a
Human Lung Microvascular Endothelial Cells (HLMVEC) provide a useful tool for studying various aspects of pathology and biology of the pulmonary microvasculature in vitro. The pulmonary vasculature is of great physiological/pathological significance. It is now recognized to be a tissue composed of metabolically active, functionally responsive cells, that interact with circulating substrates and regulate the composition of systemic arterial blood, affect target organ functions, and contribute to thrombosis, hemostasis and immune reactions, as well as tumor metastasis.
HLMVEC from Cell Applications, Inc. have been used to elucidate the therapeutic effects of Angiotensin I-converting enzyme (ACE) inhibitors, and the results revealed that they provide an additional benefit to patients by activating bradykinin B1 receptor leading to prolonged nitric oxide (NO) production in endothelial cells and inhibition of PKCϵ.
DETAILS
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Tissue
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Human lung (Nondiseased, Asthma (AS), or Type 2 Diabetes (T2D))
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QC
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No bacteria, yeast, fungi, mycoplasma, virus
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Character
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Factor VIII-related Ab, CD31 (PECAM-1), DiI-Ac-LDL uptake
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Bioassay
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Attach, spread on AFS-coated surface, proliferate in Growth Med
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Cryovial
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500,000 HLMVEC (3rd passage) frozen in Basal Medium w/ 10% FBS, 10% DMSO
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Kit
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Cryovial HLMVEC (540-05, 540AS-05, or 540T2D-05), Gr Med (111-500), Attchmnt Fctr Soln (123-100), Sbcltr Rgnt Kit (090K)
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Proliferating
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Shipped in Tsfr Med, 4th psg (flasks or plates)
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Doublings
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At least 15
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Applications
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Laboratory research use only (RUO). Not for human, clinical, diagnostic or veterinary use.
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PRODUCTS
| PRODUCT | SIZE | CAT.# |
| Cryopreserved HLMVEC, Adult: 5x10^5 Cells (Adult), Medium & Subculture Reagents (See Details tab for specifics) | 1 Kit | 540K-05a |
| Cryopreserved HLMVEC, Adult: Frozen HLMVEC (5x10^5) | 1 Cryovial | 540-05a |
| Cryopreserved HLMVEC-AS, Adult: 5x10^5 Cells (from donor with Asthma, Adult), Medium & Subculture Reagents (See Details tab for specifics) | 1 Kit | 540ASK-05a |
| Cryopreserved HLMVEC-AS, Adult: Frozen HLMVEC-AS from donor with Asthma (5x10^5) | 1 Cryovial | 540AS-05a |
| Cryopreserved HLMVEC-T2D, Adult: 5x10^5 Cells (from donor with Type 2 Diabetes, Adult), Medium & Subculture Reagents (See Details tab for specifics) | 1 Kit | 540T2DK-05a |
| Cryopreserved HLMVEC-T2D, Adult: Frozen HLMVEC-T2D from donor with Type 2 Diabetes (5x10^5) | 1 Cryovial | 540T2D-05a |
| Cryopreserved HLMVEC, Pre-Screened, Adult: 5x10^5 Cells (Pre-Screened, Adult), Medium & Subculture Reagents (See Details tab for specifics) | 1 Kit | S540K-05a |
| Cryopreserved HLMVEC, Pre-Screened, Adult: Frozen HLMVEC (5x10^5) | 1 Cryovial | S540-05a |
| Proliferating HLMVEC, Adult: Actively growing, dividing cells in medium | T-25 Flask | 541-25a |
| Proliferating HLMVEC, Adult: Actively growing, dividing cells in medium | T-75 Flask | 541-75a |
RELATED PRODUCTS
| PRODUCT | SIZE | CAT.# |
| Microvascular Endothelial Cell Growth Medium: All-in-one ready-to-use | 500 ml | 111-500 |
| Microvascular Endothelial Cell Growth Medium Kit: Basal medium & growth supplement sold together packaged separately | Yields 500 ml | 111K-500 |
| Microvascular Endothelial Cell Basal Medium: Basal medium (contains no growth supplement). Add GS before use. | 500 ml | 100-500 |
| Microvascular Endothelial Cell Growth Supplement: Added to Basal Medium to create Growth Medium | 30 ml | 111-GS |
| Human Microvascular Endothelial Cell Growth Medium Kit, separate: Basal medium & growth supplement sold, together packaged separately. Supplements in individual vials. | Yields 500 ml | 111KS-500 |
EXTENDED FAMILY PRODUCTS
| PRODUCT | SIZE | CAT.# |
| Anti-ICAM-1: Rabbit Intercellular Adhesion Molecule-1 Antibody | 100 ul | CG1238 |
| Anti-VEGF: Rabbit Vascular Endothelial Growth Factor Antibody | 100 ul | CG1634 |
| Polyclonal Vascular Endothelial Growth Factor-C Antibody: Polyclonal Vascular Endothelial Growth Factor-C Antibody | 100 ul | CB3778 |
| Polyclonal VEGF Receptor 1 Antibody: Polyclonal VEGF Receptor 1 Antibody | 100 ul | CB3839 |
| Attachment Factor Solution (AFS): AFS, contains Bovine Gelatin | 100 ml | 123-100 |
| Freezing Medium: For general cryopreservation of most primary cells. Contains FBS & DMSO. | 50 ml | 040-50 |
| Cyto-X Cell Counting Reagent: 500 tests | 1 Bottle | 028-01 |
| Cyto-X Cell Counting Reagent Sample: 100 tests | Sample | 028-S |
| Cytofect™ Endothelial Cell Transfection Kit: 250 x 24-Well Rxns | 1 Kit | TF101K |
| Cytofect™ Endothelial Cell Transfection Kit: 25 x 24-Well Rxns | 1 Sample Kit | TF101KS |
| HLMVEC RNA, Adult: Total RNA prepared from Human Lung Microvascular Endothelial Cells, adult | 10 ug | 540-R10a |
| HLMVEC RNA, Adult: Total RNA prepared from Human Lung Microvascular Endothelial Cells, adult | 25 ug | 540-R25a |
| Human Gamma-Interferon Inducible Protein 10 (IP-10 / CXCL10): Human gamma-Interferon Inducible Protein 10 | 25 ug | RP1127-25 |
| Human Gamma-Interferon Inducible Protein 10 (IP-10 / CXCL10): Human gamma-Interferon Inducible Protein 10 | 100 ug | RP1127-100 |
| Human Lung RNA: Total RNA prepared from human lung tissue | 50 ug | 1H40-50 |
| Human Gamma-Interferon Inducible Protein 10 (IP-10 / CXCL10): Human gamma-Interferon Inducible Protein 10 | 1000 ug | RP1127-1000 |
| Human Lung RNA: Total RNA prepared from human lung tissue | 250 ug | 1H40-250 |
| Subculture Reagent Kit: 100 ml each of HBSS, Trypsin/EDTA & Trypsin Neutralizing Solution | 1 Kit | 090K |
| Human Vascular Endothelial Growth Factor-121 (VEGF-121): Human Vascular Endothelial Growth Factor-121 | 10 ug | RP1116-10 |
| Human Vascular Endothelial Growth Factor-121 (VEGF-121): Human Vascular Endothelial Growth Factor-121 | 100 ug | RP1116-100 |
| Human Vascular Endothelial Growth Factor-121 (VEGF-121): Human Vascular Endothelial Growth Factor-121 | 1000 ug | RP1116-1000 |
| Human VEGF-121, Animal-Free: Human Vascular Endothelial Growth Factor-121, Animal-Free | 10 ug | RP1116AF-10 |
| Human VEGF-121, Animal-Free: Human Vascular Endothelial Growth Factor-121, Animal-Free | 100 ug | RP1116AF-100 |
| Human VEGF-121, Animal-Free: Human Vascular Endothelial Growth Factor-121, Animal-Free | 1000 ug | RP1116AF-1000 |
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