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Human Trabecular Meshwork Cells: HTMC 人骨小梁細胞

Human Trabecular Meshwork Cells: HTMC 人骨小梁細胞

產品型號： 634K-05a

Damage and dysfunction of HTMC have clinical significance. For instance, hypoxia increases DNA methylation, accompanied by altered gene expression. During the normal aging process, HTMC number decreases, and senescent cells accumulate. In glaucoma, a leading cause of irreversible blindness, decreased fluid outflow causes an elevation of intraocular pressure and progressive loss of retinal ganglion cells. Physical changes to the TM include increased fibrosis, fibronectin accumulation, and expression of ECM cross-linking enzymes. This cytoskeletal reorganization and cell loss causes the TM to become rigid and stiff. Other glaucoma-related dysfunctions include mitochondrial defects, altered signaling pathways, elevated TGF-β2, genomic DNA defects, and oxidation damage.

Myocilin expression in Trabecular Meshwork Cells was analyzed following dexamathone treatment. After cells reach 70-80% confluency, HTMC cells were treated with 100nM and 500nM of dexamethasone for 6 days in DMEM with 1% FBS. Cell lysates were analyzed for Myocilin and beta-Actin expression by Western Blot.

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規格型錄

Human Trabecular Meshwork Cells (HTMC) are endothelial-like cells in a sponge-like connective tissue located near the front of the eye. HTMC make layers of beams, part of a fibrous basement membrane containing extracellular matrix and cells. In this area of high outflow resistance, HTMC regulate eye pressure by controlling drainage of fluid into tubes that flow into the bloodstream. Outflow is mediated by alterations in contractility and tension of HTMC, which also serve as a self-cleaning filter due to their phagocytic nature. Live cell imaging of the cytoskeleton provides valuable information on actin dynamics in HTMC. Other research aims to identify treatments that relax HTMC contraction to increase fluid outflow and lower eye pressure. Specific targeting of HTMC could also play a clinical role by increasing therapeutic efficacy of nanoparticles for gene delivery.

DETAILS

Tissue	Normal healthy human adult eyes
QC	No bacteria, yeast, fungi, mycoplasma, virus
Character	Positive for Fibronectin
Bioassay	Attach, spread, proliferate in Growth Med
Cryovial	~500,000 HTMC (1st passage) in Freezing Medium w/ 10% FBS, 10% DMSO
Kit	Cryovial frozen HTMC, Growth Medium (631-500), Subculture Rgnt Kit (090K)
Proliferating	Shipped in HTMC Growth Medium, psg 2, flasks or plates
Doublings	At least 12
Applications	Laboratory research use only (RUO). Not for human, clinical, diagnostic or veterinary use.

PRODUCTS

PRODUCT	SIZE	CAT.#
Cryopreserved HTMC, Adult: 5x10^5 Cells (Adult), Medium & Subculture Reagents (See Details tab for specifics)	1 Kit	634K-05a
Cryopreserved HTMC, Adult: Frozen HTMC (5x10^5)	1 Cryovial	634-05a
Proliferating HTMC, Adult: Actively growing, dividing cells in medium	T-25 Flask	635-25a
Proliferating HTMC, Adult: Actively growing, dividing cells in medium	T-75 Flask	635-75a

RELATED PRODUCTS

PRODUCT	SIZE	CAT.#
HTMC Growth Medium: All-in-one ready-to-use	500 ml	631-500
HTMC Growth Medium Kit: Basal medium & growth supplement sold together packaged separately	Yields 500 ml	631K-500
HTMC Basal Medium: Basal medium (contains no growth supplement). Add GS before use.	500 ml	630-500
HTMC Growth Supplement: Added to Basal Medium to create Growth Medium	50 ml	631-GS

EXTENDED FAMILY PRODUCTS

PRODUCT	SIZE	CAT.#
Freezing Medium: For general cryopreservation of most primary cells. Contains FBS & DMSO.	50 ml	040-50
Cyto-X Cell Counting Reagent: 500 tests	1 Bottle	028-01
Cyto-X Cell Counting Reagent Sample: 100 tests	Sample	028-S
Subculture Reagent Kit: 100 ml each of HBSS, Trypsin/EDTA & Trypsin Neutralizing Solution	1 Kit	090K

INSTRUCTIONS HTMC

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