Human Preadipocytes: HPAd 人類前脂肪細胞：HPAd
產品型號： 802hK-05a
Human Preadipocytes (HPAd) provide ideal cell models and facilitate life science research and drug discovery for obesity, diabetes and cardiovascular diseases. We derive HPAd from human subcutaneous and heart adipose tissue and cryopreserve these fibroblast-like precursor cells at the end of primary culture. They can propagate two passages prior to differentiating into Human Adipocytes (HAd). Complete differentiation can be easily achieved through the use of Preadipocyte Differentiation Medium. Mature HAd are expected 10 days after induction of differentiation and should remain healthy and responsive for at least 2 weeks after complete differentiation. Adipose mass can be controlled by inhibition of HPAd differentiation and increase of lypolysis. HPAd are also available prescreened for expression of adipocyte markers.
HPAd from Cell Applications, Inc. have been used in multiple studies investigating the cellular basis of diabetes and obesity, as well as mechanisms of action of various drug candidates. For example, it was shown that:
- Interaction between infiltrating monocytes and adipocytes affects production of metalloproteinases and osteopontin, a proinflammatory cytokine, which ultimately leads to development of more adipose tissue and insulin resistance
- Adipocyte differentiation requires activation of Akt1 through the mTORC2-BSTA mechanism, leading to downstream suppression of FoxC2
- Instead of protecting from hyperglycemia-induced ER stress, like it does in other cells, in adipocytes a bioactive, endogenously produced compound taurine was shown to modulate the expression of adipokines under inflammatory conditions by inhibiting the STAT-3 signaling pathway (Kim, 2013a,c), and to inhibit differentiation of preadipocytes into adipocytes
- FGF21, which leads to reduction of body weight in animal models of obesity, was shown to act by modulating gene expression, phosphorylating Frs2a, Erk1/2, and Mypt1 and by increasing oxidative capacity of adipocytes via AMPK–SIRT1–PGC1a cascade
- Atrial natriuretic peptide (ANP) regulates lipid catabolism and reduce insulin resistance in HPAd by activating AMPK
- Adipocytokines are involved in normal pregnancy and pregnancy-induced hypertension
- Lotus seed extract exhibits anti-obesity and hypolipidaemic properties
- HPAd (along with our Human Dermal Fibroblasts) demonstrate the role of epigenetic modifications in increasing efficiency of iPS reprogramming
DETAILS
| Tissue |
Normal healthy human adipose tissue
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QC
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No bacteria, yeast, fungi, mycoplasma, virus |
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Character
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Positive for lipid drops in cytoplasm & Oil Red O Staining, in Differentiation Med |
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Bioassay
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Attach, spread, proliferate in Growth Med |
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Cryovial
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500,000 HPAd (2nd passage) frozen in Basal Medium w/ 20% FBS, 5% DMSO
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Kit
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Cryovial frozen HPAd (802h/s-05a), Growth Medium (811-500), Subcultr Rgnt Kit (090K)
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Proliferating
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Shipped in Gr Med, 3rd psg (flasks or plates)
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Doublings
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Can be cultured 2 passages before differentiation into HAd
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Applications
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Laboratory research use only (RUO). Not for human, clinical, diagnostic or veterinary use.
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PRODUCTS
| PRODUCT | SIZE | CAT.# |
| Cryopreserved HPAd, Heart, Adult: 5x10^5 Cells (from Heart Tissue, Adult), Medium & Subculture Reagents (See Details tab for specifics) | 1 Kit | 802hK-05a |
| Cryopreserved HPAd, Heart, Adult: Frozen HPAd from Heart Tissue (5x10^5) | 1 Cryovial | 802h-05a |
| Cryopreserved HPAd, Subcutaneous, Adult: 5x10^5 Cells (from Subcutaneous Tissue, Adult), Medium & Subculture Reagents (See Details tab for specifics) | 1 Kit | 802sK-05a |
| Cryopreserved HPAd, Subcutaneous, Adult: Frozen HPAd from Subcutaneous Tissue (5x10^5) | 1 Cryovial | 802s-05a |
| Cryopreserved HPAd-h-AS, Adult, heart: Frozen HPAd-h-AS from Heart Tissue of donor with Asthma, Adult (5x10^5) | 1 Cryovial | 802hAS-05a |
| Cryopreserved HPAd-h-AS, Adult, heart: 5x10^5 Cells (from Heart Tissue of donor with Asthma, Adult), Medium & Subculture Reagents (See Details tab for specifics) | 1 Kit | 802hASK-05a |
| Cryopreserved HPAd-h-T2D, Heart, Adult: Frozen HPAd from Heart Tissue of donor with Type 2 Diabetes (5x10^5) | 1 Cryovial | 802hT2D-05a |
| Cryopreserved HPAd-h-T2D, Heart, Adult: 5x10^5 Cells (from Heart Tissue of donor with Type 2 Diabetes, Adult), Medium & Subculture Reagents (See Details tab for specifics) | 1 Kit | 802hT2DK-05a |
| Cryopreserved HPAd-s-T2D, Subcutaneous, Adult: Frozen HPAd from Subcutaneous Tissue of donor with Type 2 Diabetes (5x10^5) | 1 Cryovial | 802sT2D-05a |
| Cryopreserved HPAd-s-T2D, Subcutaneous, Adult: 5x10^5 Cells (from Subcutaneous Tissue of donor with Type 2 Diabetes, Adult), Medium & Subculture Reagents (See Details tab for specifics) | 1 Kit | 802sT2DK-05a |
| Proliferating HPAd, Adult, heart: Actively growing, dividing cells, from heart tissue, in medium | T-25 Flask | 803h-25a |
| Proliferating HPAd, Adult, heart: Actively growing, dividing cells, from heart tissue, in medium | T-75 Flask | 803h-75a |
| Proliferating HPAd, Subcutaneous, Adult: Actively growing, dividing cells, from subcutaneous tissue, in medium | T-25 Flask | 803s-25a |
| Proliferating HPAd, Subcutaneous, Adult: Actively growing, dividing cells, from subcutaneous tissue, in medium | T-75 Flask | 803s-75a |
RELATED PRODUCTS
| PRODUCT | SIZE | CAT.# |
| HPAd Growth Medium: All-in-one ready-to-use | 500 ml | 811-500 |
| HPAd Growth Medium Kit: Basal medium & growth supplement sold together packaged separately | Yields 500 ml | 811K-500 |
| HPAd Basal Medium: Basal medium (contains no growth supplement). Add GS before use. | 500 ml | 810-500 |
| HPAd Growth Supplement: Added to Basal Medium to create Growth Medium | 50 ml | 811-GS |
EXTENDED FAMILY PRODUCTS
| PRODUCT | SIZE | CAT.# |
| Freezing Medium: For general cryopreservation of most primary cells. Contains FBS & DMSO. | 50 ml | 040-50 |
| Cytofect™ Preadipocyte Transfection Kit: 175 x 24-Well Rxns | 1 Kit | TF802K |
| Cytofect™ Preadipocyte Transfection Kit: 25 x 24-Well Rxns | 1 Sample Kit | TF802KS |
| HPAd-S RNA, Adult: Total RNA prepared from Human Preadipocytes, Subcutaneous, adult | 10 ug | 802s-R10a |
| HPAd-S RNA, Adult: Total RNA prepared from Human Preadipocytes, Subcutaneous, adult | 25 ug | 802s-R25a |
| Human Leptin: Human Leptin | 200 ug | RP1037-200 |
| Human Leptin: Human Leptin | 1000 ug | RP1037-1000 |
| Human Leptin: Human Leptin | 5000 ug | RP1037-5000 |
| Subculture Reagent Kit: 100 ml each of HBSS, Trypsin/EDTA & Trypsin Neutralizing Solution | 1 Kit | 090K |
| HPAd Differentiation Medium: Add to preadipocytes and mesenchymal stem cells to differentiate into adipocytes | 250 ml | 811D-250 |
| HPAd Differentiation Medium, Component Kit: Differentiation medium components sold together packaged separately | Yields 250 ml | 811DS-250 |
| HAd Maintenance Medium: Maintenance medium for adipocytes | 250 ml | 811M-250 |
| HAd Maintenance Medium wo Insulin: Maintenance Medium for adipocytes without insulin | 250 ml | 811Mi-250 |
| Adipoccyte Starvation Medium: Use when cells need to be starved overnight to 24 hrs before experiment | 250 ml | 811S-250 |
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