Human Prostate Epithelial Cells: HPrEpC 人前列腺上皮細胞：HPrEpC
產品型號： 934K-05a
Human Prostate Epithelial Cells (HPrEpC) are isolated from normal human prostate and provide a useful tool for studying various aspects of pathology and biology of the human prostate epithelium in vitro.
HPrEpC and Human Mammary Epithelial Cells (HMEpC), both from Cell Applications, Inc., have been used in a study demonstrating that TGF-b1 induces Smad 1/5/8 and Smad 2/3 phosphorylation and BMP signaling results only in Smad 1/5/8 phosphorylation in these primary epithelial cells, while in cancer cells BMP also elicits Smad2/3 activation, contributing to cancerogenesis.
Characterization: Morphology consistent with epithelial origin, and positive for epithelial cell marker cytokeratin 18
DETAILS
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Tissue
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Normal healthy human prostate
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QC
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No bacteria, yeast, fungi, mycoplasma, virus
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Bioassay
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Attach, spread, proliferate in Growth Med
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Cryovial
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500,000 HPrEpC (2nd passage) frozen in Basal Medium w/ 10% FBS, 10% DMSO
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Kit
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Cryovial frozen HPrEpC (934-05a), Growth Medium (215-500), Subcltr Rgnt Kit (090K)
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Proliferating
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Shipped in Tsfr Med, psg 3, flasks or plates
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Doublings
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At least 12
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Applications
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Laboratory research use only (RUO). Not for human, clinical, diagnostic or veterinary use.
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PRODUCTS
| PRODUCT | SIZE | CAT.# |
| Cryopreserved HPrEpC, Adult: 5x10^5 Cells (Adult), Medium & Subculture Reagents (See Details tab for specifics) | 1 Kit | 934K-05a |
| Cryopreserved HPrEpC, Adult: Frozen HPrEpC (5x10^5) | 1 Cryovial | 934-05a |
| Proliferating HPrEpC, Adult: Actively growing, dividing cells in medium | T-25 Flask | 935-25a |
| Proliferating HPrEpC, Adult: Actively growing, dividing cells in medium | T-75 Flask | 935-75a |
RELATED PRODUCTS
| PRODUCT | SIZE | CAT.# |
| Human Epi Basal Medium: Basal medium (contains no growth supplement). Add GS before use. | 500 ml | 214-500 |
| Human Epi Growth Medium for HPrEpC & HPlEpC: All-in-one ready-to-use | 500 ml | 215-500 |
| Human Epi Growth Medium Kit for HPrEpC & HPlEpC: Basal medium & growth supplement sold together packaged separately | Yields 500 ml | 215K-500 |
| Human Epi Growth Supplement for HPrEpC & HPlEpC: Added to Basal Medium to create Growth Medium | 5 ml (3 parts) | 215-GS |
| Human EpiVita Basal Medium: Basal medium (contains no growth supplement). Add GS before use. | 500 ml | 140-500 |
EXTENDED FAMILY PRODUCTS
| PRODUCT | SIZE | CAT.# |
| Monoclonal IL-1 Receptor Antagonist Antibody: Monoclonal IL-1 Receptor Antagonist Antibody | 100 ul | CB19846 |
| Freezing Medium: For general cryopreservation of most primary cells. Contains FBS & DMSO. | 50 ml | 040-50 |
| Cyto-X Cell Counting Reagent: 500 tests | 1 Bottle | 028-01 |
| Cyto-X Cell Counting Reagent Sample: 100 tests | Sample | 028-S |
| Cytofect™ Epithelial Cell Transfection Kit: 124 x 24-Well Rxns | 1 Kit | TF102K |
| Cytofect™ Epithelial Cell Transfection Kit: 25 x 24-Well Rxns | 1 Sample Kit | TF102KS |
| Human Epidermal Growth Factor (EGF): Human Epidermal Growth Factor | 100 ug | RP1026-100 |
| Human Epidermal Growth Factor (EGF): Human Epidermal Growth Factor | 500 ug | RP1026-500 |
| Human Epidermal Growth Factor (EGF): Human Epidermal Growth Factor | 1000 ug | RP1026-1000 |
| HPrEpC RNA, Adult: Total RNA prepared from Human Prostate Epithelial Cells, adult | 10 ug | 934-R10a |
| HPrEpC RNA, Adult: Total RNA prepared from Human Prostate Epithelial Cells, adult | 25 ug | 934-R25a |
| Human Interleukin-1-Alpha (IL-1 alpha): Human Interleukin-1-alpha | 10 ug | RP1173-10 |
| Human Interleukin-1-Alpha (IL-1 alpha): Human Interleukin-1-alpha | 100 ug | RP1173-100 |
| Human Interleukin-1-Alpha (IL-1 alpha): Human Interleukin-1-alpha | 1000 ug | RP1173-1000 |
| Human Prostate RNA: Total RNA prepared from human prostate tissue | 50 ug | 1H52-50 |
| Human Prostate RNA: Total RNA prepared from human prostate tissue | 250 ug | 1H52-250 |
| Subculture Reagent Kit: 100 ml each of HBSS, Trypsin/EDTA & Trypsin Neutralizing Solution | 1 Kit | 090K |
| Human EGF, Animal-Free: Human Epidermal Growth Factor, Animal-Free | 100 ug | RP1026AF-100 |
| Human EGF, Animal-Free: Human Epidermal Growth Factor, Animal-Free | 500 ug | RP1026AF-500 |
| Human EGF, Animal-Free: Human Epidermal Growth Factor, Animal-Free | 1000 ug | RP1026AF-1000 |
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