Bovine Brain Microvascular Endothelial Cells: BBMVEC 牛腦微血管內皮細胞：BBMVEC
產品型號： B840K-05
Bovine Brain Microvascular Endothelial Cells (BBMVEC) provide an excellent model system to study many aspects of endothelial function and disease, especially those related to the blood-brain barrier (BBB).
BBMVEC from Cell Applications, Inc. have been utilized extensively in research, for example to:
- Show that alkalosis activates ERK in aortic, but not brain microvascular endothelial cells
- Study the mechanisms of blood-brain barrier (BBB) penetration by fungal pathogens during invasion
- Demonstrate opposite effects of osteoponin isoforms on angiogenesisin
- Develop an in vitro capillary assay and show VEGF secretion by endothelial cells
- Investigate the mechanisms of accumulation and effects of amyloid deposits on brain vasculature in cerebral amyloid angiopathy
- Improve drug loading and delivery stealth dendrimer carriers
- Report that the BBB breaks down under hypoxic conditions
- In indicate that increased contractility and oxidative stress are involved in development of post-stroke brain edema
- Exhibit blood-brain barrier (BBB) function can result from shear stress, acting through a pathway that upregulates key factors and increases their localization to tight junctions
- Demonstrate that brain microvascular endothelial cells show higher sensitivity to oxidative stress generated by advanced glycation end products due to stronger VEGF expression leading to increased permeability
- Show, along with Bovine Aortic Endothelial Cells, that brain microvasculature is more sensitive to pathogenesis, compared to large vessel endothelia,
- Demonstrate that C-reactive protein (CRP), a cardiovascular risk factor, induces higher oxidative stress in the brain microvasculature
- Support the key role of ROS by showing that activation of antioxidant genes by Nrf2 reduces brain vascular leak from acute high altitude exposure known to induce ROS
- Determine that IL-1β, ZYM, and LTA increase the permeability of the BBB to small ions, while TNF-α and lipopolysaccharide disrupt the endothelial layer integrity to allow passage of larger molecules
- Investigate the role of basolateral environment in modulating BBB by regulating expression and biochemical properties of the tight junction proteins, occludin and ZO-1
- Show that during cerebral ischemia increased expression of TWEAK and Fn14 in the endothelial-astrocyte interface facilitating leukocyte transmigration and recruitment to the ischemic tissue
- Demonstrate that apigenin, a dietary flavonoid, activates Ca2+-activated K+ channels in endothelial cells leading to a hyperpolarization followed by a Ca2+ influx causing increased NO production followed by Akt dephosphorylation
- Develop gene and drug delivery methods for crossing the BBB based on polymer-based nanoparticles or adenovirus or gold nanoparticles modified to be transported via transcytosis pathway
DETAILS
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Tissue
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Normal healthy bovine brain microvessels
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QC
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No bacteria, yeast, fungi, mycoplasma
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Character
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DiI-Ac-LDL uptake: Positive
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Bioassay
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Attach, spread on Attachment Factor-coated surface, proliferate in Growth Med
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Cryovial
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500,000 BBMVEC (2nd passage) frozen in Basal Medium w/ 10% FBS, 10% DMSO
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Kit
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Cryovial frozen BBMVEC (B840-05), Gr Med (B819-500), Attchmnt Fctr Soln (123-100), Subcltr Rgnt Kit (090K)
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Proliferating
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Shipped in Gr Med, 3rd psg (flasks or plates)
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Doublings
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At least 12
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Applications
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Laboratory research use only (RUO). Not for human, clinical, diagnostic or veterinary use.
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PRODUCTS
| PRODUCT | SIZE | CAT.# |
| Cryopreserved BBMVEC: 5x10^5 Cells, Medium & Subculture Reagents (See Details tab for specifics) | 1 Kit | B840K-05 |
| Cryopreserved BBMVEC: Frozen BBMVEC (5x10^5) | 1 Cryovial | B840-05 |
| Proliferating BBMVEC: Actively growing, dividing cells in medium | T-25 Flask | B841-25 |
| Proliferating BBMVEC: Actively growing, dividing cells in medium | T-75 Flask | B841-75 |
RELATED PRODUCTS
| PRODUCT | SIZE | CAT.# |
| Bovine Brain EC Basal Medium: Basal medium (contains no growth supplement). Add GS before use. | 500 ml | B818-500 |
| Bovine Brain EC Growth Medium: All-in-one ready-to-use | 500 ml | B819-500 |
| Bovine Brain EC Growth Medium Kit: Basal medium & growth supplement sold together packaged separately | Yields 500 ml | B819K-500 |
| Bovine Brain EC Growth Supplement: Added to Basal Medium to create Growth Medium | 50 ml x 2 | B819-GS |
EXTENDED FAMILY PRODUCTS
| PRODUCT | SIZE | CAT.# |
| BBMVEC RNA : Total RNA prepared from Bovine Brain Microvascular Endothelial Cells | 10 ug | B840-R10 |
| BBMVEC RNA : Total RNA prepared from Bovine Brain Microvascular Endothelial Cells | 25 ug | B840-R25 |
| Freezing Medium: For general cryopreservation of most primary cells. Contains FBS & DMSO. | 50 ml | 040-50 |
| Cytofect™ Endothelial Cell Transfection Kit: 250 x 24-Well Rxns | 1 Kit | TF101K |
| Cytofect™ Endothelial Cell Transfection Kit: 25 x 24-Well Rxns | 1 Sample Kit | TF101KS |
| Subculture Reagent Kit: 100 ml each of HBSS, Trypsin/EDTA & Trypsin Neutralizing Solution | 1 Kit | 090K |
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