Normal Human Epidermal Keratinocytes 表皮角質形成細胞

產品型號: FC-0007_FC-0025_FC-0064

Lifeline® Normal Human Epidermal Keratinocytes neonatal (HEKn), when grown in Lifeline DermaLife K Medium, provides an ideal serum-free culture model, for the study of wound healing, toxicology or epithelial biology.

Lifeline Epidermal Keratinocytes are cryopreserved as primary cells to ensure the highest viability and plating efficiency.  Cells are isolated from neonatal human foreskin and expanded once in culture vessels before cryopreservation.

  • Our Neonatal Epidermal Keratinocytes are quality tested in DermaLife K Medium to ensure optimal serum-free growth over a period of at least 15 population doublings at rates equal to or greater than serum-supplemented medium.
  • Keratinocytes can be grown without serum, phenol red or antimicrobials when cultured in DermaLife medium.

Lifeline® Epidermal Keratinocytes need not be exposed to antimicrobials or phenol red when cultured in DermaLife medium, an advantage since these supplements can cause cell stress and “masking effects” that may negatively impact experimental results. Lifeline® offers these traditional supplements, but they are not needed or recommended to achieve optimal cell performance in most research applications.

 

 

keratinocytes culture

Quality Testing for Guaranteed Consistency and Reproducible Results

Lifeline® Cell Technology manufactures products using the highest quality raw materials and incorporates extensive quality assurance in every production run. Exacting standards and production procedures ensure consistent performance.

500,000 cells per vial

 

Cell Count  500,000 cryopreserved cells per vial
Proliferation and Morphology Normal cell appearance for 15 population doublings
Cell Viability Minimum 50% viability when thawed from cryopreservation
Sterility

Testing Negative for mycoplasma

Negative for bacterial and fungal growth for 14 days

Virus Testing Negative for HIV-1, HIV-2, HBV, and HCV by PCR

 

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