Renal Proximal Tubule Epithelial Cells 人類近端腎小管上皮細胞

產品型號: FC-0013

Lifeline® normal Human Primary Renal Proximal Tubule Epithelial Cells, when grown in Lifeline RenaLife™ Medium, provide an ideal cell system to study of renal function, fibrosis, inflammation, metabolism, nephrotoxicity or cancer.  Additionally, our normal human renal proximal tubule cells can be used for drug development or screening.

  • Lifeline® renal cells can be used in high throughput assays, such as cytotoxicity and drug screening assays. Customers that use our renal cells for these types of assays report high reproducibility with coefficients of variability below 10% (usually 4-8%).

Lifeline® Renal Proximal Tubule Epithelial Cells are isolated from human kidney tissue.

  • The cells have been isolated, plated and expanded in culture vessels before being harvested for cryopreservation to ensure the highest purity, viability and plating efficiency.

Our Renal Epithelial Cells are quality tested in RenaLife™ Medium to ensure optimal reduced-serum growth and maintenance of morphology over a period of at least 15 population doublings at rates equal to or greater than serum-supplemented media.

  • Renal Proximal Tubule Epithelial Cells can be grown in a 0.5% serum medium without phenol red or antimicrobials when cultured in RenaLife™ Medium.
Lifeline® Human Renal Proximal Tubule cells are not exposed to antimicrobials or phenol red when cultured in
Lifeline® RenaLife™ medium, an advantage since these supplements can cause cell stress and “masking effects”
that may negatively impact experimental results. Lifeline® offers these traditional supplements; however, they are
not needed, or recommended, to achieve optimal cell performance in most research applications.

500,000 cells per vial

 

NORMAL HUMAN ENDOTHELIAL CELLS ARE TESTED FOR:

 

Cell Count  500,000 cryopreserved cells per vial
Proliferation and Morphology Normal cell appearance for 15 population doublings
Cell Viability Minimum 50% viability when thawed from cryopreservation
Sterility

Testing Negative for mycoplasma

Negative for bacterial and fungal growth

Virus Testing Negative for HIV-1, HIV-2, HBV, and HCV by PCR
Specific Enzyme Test

Renal Proximal Tubule cells have Y-glutamyl transferase activity

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