Human Coronary Artery Endothelial Cells 人體冠狀動脈內皮細胞

Lifeline® Normal Human Coronary Artery Endothelial Cells (HCAEC), when grown in Lifeline® VascuLife® Medium, provides an ideal culture model, with or without human VEGF, for the study of angiogenesis, atherosclerosis or vascular biology.

• Coronary Artery Endothelial Cells are isolated in VascuLife® EnGS Medium to ensure that they remain unexposed to recombinant human VEGF.
• Lifeline® Coronary Artery Endothelial Cells may be cultured with or without VEGF.
• Coronary Artery Endothelial Cells can be grown in low serum (2%) medium without phenol red or antimicrobials when cultured in VascuLife® medium.

Lifeline® HCAEC are isolated from human coronary arteries. The cells have been isolated, plated and expanded in culture vessels three times before being harvested for cryopreservation to ensure the highest viability and plating efficiency.

• Our HCAEC are quality tested in VascuLife® EnGS medium to ensure optimal reduced-serum growth over a period of at least 15 population doublings at rates equal to or greater than other serum-supplemented medium.
• Human Coronary Artery Endothelial Cells are not exposed to antimicrobials or phenol red when cultured in VascuLife® medium, an advantage since these supplements can cause cell stress and “masking effects” that may negatively impact experimental results.

500,000 cells per vial

Lifeline® Coronary Artery Endothelial Cells have been quality tested for:

• Proliferation and Morphology:  Normal cell appearance for 15 population doublings
• Cell Viability:  Minimum 70% viability when thawed from cryopreservation
• Sterility:  Negative for mycoplasma. Negative for bacterial and fungal growth
• Virus:  Negative for HIV-1, HIV-2, HBV, and HCV by PCR
• Specific Staining:  von Willebrand Factor positive. Smooth muscle a-actin negative.