Human Bronchial/Tracheal Epithelial Cells, Primary 人體支氣管/氣管上皮細胞, 原代

Lifeline® Normal Human Bronchial/Tracheal Epithelial Cells (HBTEC), when grown in Lifeline® BronchiaLife™ Medium, provide an ideal serum-free culture model for the study of respiratory infection, asthma, chronic obstructive pulmonary disease, cystic fibrosis, response to inflammation, and the damaging effects of smoking.

HBTEC are cryopreserved as primary cells, e.g., cells are isolated from human bronchi/trachea and expanded once in culture vessels before being harvested for cryopreservation.

Lifeline® HBTEC are cultured without retinoic acid and cryopreserved as primary cells to ensure the highest viability and plating efficiency. Our HBTEC are quality tested in BronchiaLife™ Medium to ensure optimal growth over a period of at least 15 population doublings at rates equal to or greater than other commercially available media.

HBTEC are not exposed to antimicrobials or phenol red when cultured in BronchiaLife™ medium, an advantage since these supplements can cause cell stress and “masking effects” that may negatively impact experimental results.

Normal Human Epithelial Cells are tested for:

• Cell Count: ≥ 500,000 cryopreserved cells per vial
• Proliferation and Morphology: Normal cell appearance for 15 population doublings
• Cell Viability: Minimum 70% viability when thawed from cryopreservation
• Sterility: Negative for mycoplasma, bacterial, and fungal growth
• Virus: Negative for HIV-1, HIV-2, HBV, and HCV by PCR