Porcine Corneal Keratocytes: PCK 豬角膜角質細胞：PCK
產品型號： P632K-05
Porcine Corneal Keratocytes (PCK) are primary cells derived from porcine cornea. Corneal Keratocytes are neural-crest derived mesenchymal cells that populate the corneal stroma. The stroma is comprised of a type I and type V collagen fibers arranged as lamellae, makes up majority of the corneal thickness responsible for promoting corneal transparency. Upon corneal injury, keratocytes are stimulated to either undergo apoptosis or transition into repair phenotypes to promote corneal regeneration.
Porcine Corneal Keratocytes cultured in Corneal Keratocyte Growth Medium are able to propagate to 10 population doublings when cultured under the recommended conditions. PCK could be used as an important in vitro cell model to study keratocyte differentiation, corneal injury and related diseases.
DETAILS
| Tissue | Normal healthy porcine cornea |
| QC | No bacteria, yeast, fungi, mycoplasma, virus |
| Bioassay | Attach, spread, proliferate in Growth Med |
| Cryovial | 500,000 PCK (1st passage) frozen in Basal Medium w/ 10% FBS, 10% DMSO |
| Kit | Cryovial frozen PCK (P632-05), Growth Medium (P6111-500), Subculture Rgnt Kit (090K) |
| Proliferating | Shipped in Tsfr Med, psg 1, flasks or plates |
| Doublings | At least 15 |
| Applications | Laboratory research use only (RUO). Not for human, clinical, diagnostic or veterinary use. |
PRODUCTS
| PRODUCT | SIZE | CAT.# |
| Cryopreserved PCK: 5x10^5 Cells, Medium & Subculture Reagents (See Details tab for specifics) | 1 Kit | P632K-05 |
| Cryopreserved PCK: Frozen PCK (5x10^5) | 1 Cryovial | P632-05 |
| Proliferating PCK: Actively growing, dividing cells in medium | T-25 Flask | P633-25 |
| Proliferating PCK: Actively growing, dividing cells in medium | T-75 Flask | P633-75 |
RELATED PRODUCTS
| PRODUCT | SIZE | CAT.# |
| PCK Growth Medium: All-in-one ready-to-use | 500 ml | P6111-500 |
| PCK Growth Medium Kit: Basal medium & growth supplement sold together packaged separately | 500 ml | P6111K-500 |
| PCK Basal Medium: Basal medium (contains no growth supplement). Add GS before use. | 500 ml | P6110-500 |
| PCK Growth Medium Supplement: Added to Basal Medium to create Growth Medium | 50 ml | P6111-GS |
EXTENDED FAMILY PRODUCTS
| PRODUCT | SIZE | CAT.# |
| Cyto-X Cell Counting Reagent: 500 tests | 1 Bottle | 028-01 |
| Cyto-X Cell Counting Reagent Sample: 100 tests | Sample | 028-S |
| Freezing Medium: For general cryopreservation of most primary cells. Contains FBS & DMSO. | 50 ml | 040-50 |
| Subculture Reagent Kit: 100 ml each of HBSS, Trypsin/EDTA & Trypsin Neutralizing Solution | 1 Kit | 090K |
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