Rat Epidermal Keratinocytes: REK 大鼠表皮角質形成細胞：REK
產品型號： R102K-05n
Rat Epidermal Keratinocytes (REK) from Cell Applications, Inc. provide a good model system to study many aspects of epithelial function and disease, particularly those related to skin biology and toxicology.
- It was shown that TGFβ1 treatment decreased c-Myc mRNA in REK which, taken together with the TGFβ1-induced growth arrest exhibited by keratinocytes and opposite results obtained in other cells, suggests a correlation between c-Myc mRNA expression and the mitogenic response.
- Additionally, REK were used to demonstrate that pathological increases in keratinocyte sodium channels Nav1.1, Nav1.6, and Nav1.8 expression may contribute to pain by increasing epidermal ATP release, resulting in excessive activation of P2X receptors on primary sensory axons.
- REK were also used to demonstrate that laminin 332 deposition is inhibited by ionizing radiation and, in combination with slower keratinocyte migration, can contribute to the delayed wound healing of irradiated skin.
DETAILS
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Tissue
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Normal healthy rat skin
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QC
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No bacteria, yeast, fungi, mycoplasma
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Bioassay
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Attach, spread, proliferate in Growth Med
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Cryovial
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500,000 REK (primary culture) frozen in Basal Medium w/ 10% FBS, 10% DMSO
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Kit
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Cryovial frozen REK (R102-05n), Growth Medium (R131-250)
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Proliferating
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Shipped in Gr Med, 1st psg (flasks or plates)
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Doublings
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REK do not expand and cannot be subcutured. Thaw & plate REK directly to assay format. REK will undergo only 1 or 2 doublings.
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Applications
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Laboratory research use only (RUO). Not for human, clinical, diagnostic or veterinary use.
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PRODUCTS
| PRODUCT | SIZE | CAT.# |
| Cryopreserved REK, Neonatal: 5x10^5 Cells (Neonatal) & Medium (See Details tab for specifics) | 1 Kit | R102K-05n |
| Cryopreserved REK, Neonatal: Frozen REK (5x10^5) | 1 Cryovial | R102-05n |
| Proliferating REK, Neonatal: Actively growing, dividing cells, in medium | T-25 Flask | R103-25n |
| Proliferating REK, Neonatal: Actively growing, dividing cells, in medium | T-75 Flask | R103-75n |
| Cryopreserved REK, Adult: Frozen REK (5x10^5) | 1 Cryovial | R102-05a |
| Cryopreserved REK, Adult: 5x10^5 Cells (Adult) & Medium (See Details tab for specifics) | 1 Kit | R102K-05a |
| Proliferating REK, Adult: Actively growing, dividing cells, in medium | T-25 Flask | R103-25a |
| Proliferating REK, Adult: Actively growing, dividing cells, in medium | T-75 Flask | R103-75a |
RELATED PRODUCTS
| PRODUCT | SIZE | CAT.# |
| REK Basal Medium: Basal Medium without Growth Supplement | 250 ml | R130-250 |
| REK Growth Medium: All-in-one ready-to-use media | 250 ml | R131-250 |
| REK Growth Medium Kit: Basal medium & growth supplement sold together packaged separately | Yields 250 ml | R131K-250 |
| REK Growth Supplement: Added to Basal Medium to create Growth Medium | 1 ml (2 parts) | R131-GS |
| Cyto-X Cell Counting Reagent Sample: 100 tests | Sample | 028-S |
| Cyto-X Cell Counting Reagent: 500 tests | 1 Bottle | 028-01 |
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