Human Epidermal Melanocytes: HEM人類表皮黑色素細胞:HEM

產品型號: 104K-05a

Human Epidermal Melanocytes (HEM) from Cell Applications, Inc. maintain their characteristic shape in culture for many generations.  They produce melanin and serve as a useful cell model for the studies of melanocyte proliferation and differentiation, as well as progression of melanocytic neoplasia. Epidermal melanocytes are pigment-producing cells located at the basal level of epidermis, where they interact with keratinocytes via cellular processes called dendrites.  Melanin, the pigment produced by melanocytes and responsible for skin color, is then transferred to keratinocytes, where it is stored in vesicles called melanosomes located around the nucleus to provide protection from UV radiation.
HEM provided by Cell Applications, Inc. have been used to:
  • Identify unique features and biomarkers of melanoma cells
  • Show that IGFBP7 is dispensable for B-RAFV600E-induced senescence
  • Investigate mechanisms of cellular senescence, in particular by showing that Id1 extends the life span of melanocytes through inhibition of p16/INK4a expression
  • Discover the central role of oncogenic BRAF gene in melanoma oncogenesis by demonstrating that the constitutive MAPK pathway activation leading to activation of mTOR, STAT3-dependent transcription of Mcl-1, Tbx3-mediated repression of E-cadherin leading to increased metastasis in melanoma cells all result from overexpression and/or mutations of BRAF gene
  • Discover other key players in oncogenic signaling leading to melanoma, such as PI3K which regulates MAPK activation in response to oncogenic c-Kit activity, Nck2 adaptor protein which participates in  regulation of tyrosine kinases activity and the role of mitochondria metabolism in advanced melanoma
  • Demonstrate that p16INK4a-Rb-CDK4/6 senescence-inducing tumor suppressor pathway in inhibited in melanoma cells leading to proliferation of cells harboring DNA damage and by studying expression of senescence markers

DETAILS

Tissue
Normal healthy human neonatal foreskin
QC
No bacteria, yeast, fungi, mycoplasma, virus
Bioassay
Attach, spread, proliferate in Growth Med
Cryovial
500,000 HEM (2nd passage) frozen in Basal Medium w/ 30% FBS, 10% DMSO
Kit 
Cryovial frozen HEM (104-05n), Growth Medium (135-500), Subcltr Rgnt Kit (090K)
Proliferating
Shipped in Gr Med, 3rd psg (flasks or plates)
Doublings
At least 12
Applications
Laboratory research use only (RUO). Not for human, clinical, diagnostic or veterinary use.

 

PRODUCTS

Product Size CAT.#
Cryopreserved Cryopreserved HEM, NeonatalHuman Epidermal Melanocytes Total Kit, neonatal: 5x10^5 Cells (Neonatal), Medium & Subculture Reagents (See Details tab for specifics) 1 Kit 104K-05n
Cryopreserved Epidermal Melanocyte (HEM), neonatal: Frozen HEM (5x10^5) 1 Cryovial 104-05n
Cryopreserved Human Epidermal Melanocytes Total Kit, adult: 5x10^5 Cells (Adult), Medium & Subculture Reagents (See Details tab for specifics) 1 Kit 104K-05a
Cryopreserved Epidermal Melanocyte (HEM), adult: Frozen HEM (5x10^5) 1 Cryovial 104-05a

RELATED PRODUCTS

Product Size CAT.#
HEM Growth Medium: All-in-one ready-to-use 500 ml 135-500
HEM Growth Medium Kit: Basal medium & growth supplement sold together packaged separately Yields 500 ml 135K-500
HEM Basal Medium: Basal medium (contains no growth supplement).  Add GS before use. 500 ml 134-500
HEM Growth Supplement: Added to Basal Medium to create Growth Medium 5 ml (3 parts) 135-GS
Freezing Medium for HEM: Freezing Medium optimized for the unique needs of Human Epidermal Melanocytes (HEM). 50 ml 040-50HEM

 

EXTENDED FAMILY PRODUCTS

 

Product Size CAT.#
Human Epidermal Growth Factor (EGF): Human Epidermal Growth Factor 100 ug RP1026-100
Human Epidermal Growth Factor (EGF): Human Epidermal Growth Factor 500 ug RP1026-500
Human Epidermal Growth Factor (EGF): Human Epidermal Growth Factor 1000 ug RP1026-1000
Epidermal Melanocyte RNA (HEM RNA), Neonatal: Total RNA prepared from Human Epidermal Melanocytes, neonatal 10 ug 104-R10n
Epidermal Melanocyte RNA (HEM RNA), Neonatal: Total RNA prepared from Human Epidermal Melanocytes, neonatal 25 ug 104-R25n
Subculture Reagent Kit: 100 ml each of HBSS, Trypsin/EDTA & Trypsin Neutralizing Solution 1 Kit 090K
Human EGF, Animal-Free: Human Epidermal Growth Factor, Animal-Free 100 ug RP1026AF-100
Human EGF, Animal-Free: Human Epidermal Growth Factor, Animal-Free 500 ug RP1026AF-500
Human EGF, Animal-Free: Human Epidermal Growth Factor, Animal-Free 1000 ug RP1026AF-1000
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