Human Umbilical Vein Endothelial Cells: HUVEC 人類臍靜脈內皮細胞:HUVEC
產品型號: 200K-05f
Human Umbilical Vein Endothelial Cells (HUVEC) provide a classic model system to study many aspects of endothelial function and disease, such as normal, abnormal and tumor-associated angiogenesis, oxidative stress, hypoxia and inflammation related pathways in endothelia under normal and pathological conditions, cardiovascular-related complications associated with various diseases, mode of action and cardiovascular protection effects of various compounds, etc.
Select HUVEC lots have been tested to demonstrate stimulation-dependent angiogenesis and key endothelial cell signaling pathways (phosphorylation of VEGFR2). More information about pre-screened endothelial cells can be found on the Pre-Screened Endothelial Cell Product Page.
HUVEC from Cell Applications, Inc. have been utilized in numerous research publications, for example, to:
- Understand anti-inflammatory properties of HDL
- Develop scaffolds for tissue engineering
- Characterize endothelial cell response to stress and discover mechanisms that protect mitochondria against oxidative stress
- Demonstrate that prolonged hypoxia induces MMP-1 expression, EC migration and angiogenesis
- Show that Alzheimer’s β-amyloid peptide exhibits anti-angiogenic properties
- Demonstrate for the first time that mature miRNA can control gene expression in a cell where it is neither transcribed nor processed
- Show that abnormal matrix composition characteristic for systemic sclerosis leads to impaired vascular function and angiogenesis
- Discover novel mechanisms of inhibiting tumor angiogenesis and metastasis, by limiting EC migration and proliferation, as well as the expression of growth factors and enzymes
- Develop a bubble lyposome based system that enables therapeutic miRNA delivery
- Demonstrate that commercially used flame retardants cause oxidative stress
- Show that hyperglycemia leads to endothelial dysfunction, partially due to depletion of antioxidants, and that regaining glycemic control normalizes growth factor levels in ischemia and improves perfusion recovery
- Show that inhibition of aldose reductase inhibits migration and formation of capillary-like structures by endothelial cells
- Investigate effects of molecular mobility of the outmost material surfaces on cellular adhesion and organization
- Identify signaling differences in cells cultured on different ECM components.
- Investigate mechanisms of cardiovascular protection exerted by bioactive plant and fungal components
- Show that in low doses coumaric acid and resveratrol provide beneficial effects and protect endothelial cells from reactive oxygen species
- Show that combined treatment with melatonin and atorvastatin reversed damage to EC, partly by reducing free radical generation and lipid peroxidation
- Discover that deferoxamine (DFO), an iron-chelating agent, mitigates deleterious effects of radiation on angiogenesis
- Study the differential effects of wood smoke and diesel exhaust particles, as well as carbon nanotubes on inducing oxidative stress and production of cytokines and adhesion molecules in endothelia
DETAILS
Tissue
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Healthy human umbilical vein
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QC
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No bacteria, yeast, fungi, mycoplasma, virus
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Bioassay
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Attach, spread, proliferate in Growth Med
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Cryovial
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500,000 HUVEC (primary culture) in Bas Med w/ 10% FBS, 10% DMSO
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Kit
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Cryovial frozen HUVEC (200-05n), Growth Medium (211-500), Subculture Rgnt Kit (090K)
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Proliferating
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Shipped in Tsfr Med, 1st psg (flasks or plates)
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Doublings
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At least 16
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Applications
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Laboratory research use only (RUO). Not for human, clinical, diagnostic or veterinary use.
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PRODUCTS
Product | Size | CAT.# |
Cryopreserved Human Umbilical Vein Endothelial Cells Total Kit, neonatal: 5x10^5 Cells (Neonatal), Medium & Subculture Reagents (See Details tab for specifics) | 1 Kit | 200K-05n |
Cryopreserved Umbilical Vein Endothelial Cells (HUVEC), neonatal: Frozen HUVEC (5x10^5) | 1 Cryovial | 200-05n |
Cryopreserved Human Umbilical Vein Endothelial Cells, Pooled, Total Kit, neonatal: 5x10^5 Cells (Pooled, Neonatal), Medium & Subculture Reagents (See Details tab for specifics) | 1 Kit | 200pK-05n |
Cryopreserved Umbilical Vein Endothelial Cells (P-HUVEC), Pooled, neonatal: Frozen HUVEC, pooled (5x10^5) | 1 Cryovial | 200p-05n |
Cryopreserved Pre-Screened Cryopreserved S-HUVEC Total Kit, neonatal: 5x10^5 Cells (Pre-Screened, Neonatal), Medium & Subculture Reagents (See Details tab for specifics) | 1 Kit | S200K-05n |
Cryopreserved Umbilical Vein Endothelial Cells (HUVEC), Pre-Screened, neonatal : Frozen HUVEC (5x10^5) | 1 Cryovial | S200-05n |
RELATED PRODUCTS
Product | Size | CAT.# |
Human EC Growth Medium: All-in-one ready-to-use. Optimized and best-suited for angiogenesis and other physiological studies. For accelerated growth, use Cat# 213-500. | 500 ml | 211-500 |
Human EC Growth Medium Kit: Basal medium & growth supplement sold together packaged separately. Optimized and best-suited for angiogenesis and other physiological studies. For accelerated growth, use Cat# 213-500. | Yields 500 ml | 211K-500 |
Human EC Basal Medium: Basal medium (contains no growth supplement). Add GS before use. | 500 ml | 210-500 |
Human EC Growth Supplement: Added to Basal Medium to create Growth Medium. The complete Growth Medium (equivalent to Cat# 211-500) is optimized and best-suited for angiogenesis and other physiological studies. For accelerated growth, use Cat# 213-500. | 15 ml | 211-GS |
Human EC Growth Medium V2: All-in-one ready-to-use. Enriched with more growth factors for accelerated cell growth. For angiogenesis studies, use Cat# 211-500. | 500 ml | 213-500 |
Endothelial Cells Growth Medium V2 Kit, 500ml: Basal medium & growth supplement sold together packaged separately. Enriched with more growth factors for accelerated cell growth. For angiogenesis studies, use Cat# 211K-500. | 500 ml | 213K-500 |
Human EC Serum-Free Defined Medium: Defined medium without serum designed to maintain endothelial cell monlayers in confluent cultures. Not for cell growth. | 500 ml | 113-500 |
Human EC Starvation Medium: Use when cells need to be starved overnight to 24 hrs before experiment | 250 ml | 209-250 |
Human EC Growth Medium wo Phenol Red: Growth medium without phenol red. Optimized and best-suited for angiogenesis and other physiological studies. For accelerated growth, use Cat# 213-500. | 500 ml | 211PR-500 |
Human EC Growth Medium wo FBS: 211-500 growth medium, but without FBS. FBS must be added in order to support cell culture. | 500 ml | 211F-500 |
Human EC Growth Medium wo Antibiotics: Growth medium without antibiotics. Optimized and best-suited for angiogenesis and other physiological studies. Suitable for Cytofect Transfection Kit. For accelerated growth, use Cat# 213-500. | 500 ml | 211A-500 |
Human EC Growth Medium wo Hydrocortisone: Growth medium without hydrocortisone. The complete Growth Medium is optimized and best-suited for angiogenesis and other physiological studies. For accelerated growth, use Cat# 213-500. | 500 ml | 211H-500 |
Human EC Growth Supplement wo FBS: Growth supplement without FBS. FBS must be added in order to support cell culture. | 5 ml | 211F-GS |
Human EC Growth Medium Kit V2, components separate: Basal medium & growth supplement sold together packaged separately. Supplements in individual vials. Enriched with more growth factors for accelerated cell growth. For angiogenesis studies, use Cat# 211K-500. | 500 ml | 213KS-500 |
Human EC Growth Supplement wo Antibiotics: Growth supplement without antibiotics | 15 ml | 211A-GS |
Human EC Growth Supplement wo Hydrocortisone: Growth supplement without hydrocortisone | 15 ml | 211H-GS |
Human EC Serum-Free Defined Medium wo Phenol Red: Defined medium, without serum or phenol red, designed to maintain endothelial cell monlayers in 100% confluent culture. Not intended for cell growth. | 500 ml | 113PR-500 |
Human EC Starvation Medium wo Phenol Red: Starvation medium without phenol red | 250 ml | 209PR-250 |
HUVEC Conditioned Medium: HUVEC Conditioned Medium | 5 ml | 211CM-05 |
EXTENDED FAMILY PRODUCTS
Product | Size | CAT.# |
Freezing Medium: For general cryopreservation of most primary cells. Contains FBS & DMSO. | 50 ml | 040-50 |
Cytofect HUVEC Transfection Kit (250 x 24-Wells): 250 x 24-Well Rxns | 1 Kit | TF200K |
Cytofect HUVEC Transfection Sample Kit (25 x 24-Wells): 25 x 24-Well Rxns | 1 Sample Kit | TF200KS |
Human E-Selectin ELISA Kit: Human E-Selectin ELISA Kit | 96 Wells | CL0501 |
Umbilical Vein Endothelial Cell RNA (HUVEC RNA), Stimulated RNA, Neonatal: Total RNA prepared from Human Umbilical Vein Endothelial Cells, Stimulated, neonatal | 10 ug | 200S-R10n |
Umbilical Vein Endothelial Cell RNA (HUVEC RNA), Stimulated RNA, Neonatal: Total RNA prepared from Human Umbilical Vein Endothelial Cells, Stimulated, neonatal | 25 ug | 200S-R25n |
Umbilical Vein Endothelial Cell RNA (HUVEC RNA), Neonatal: Total RNA prepared from Human Umbilical Vein Endothelial Cells, neonatal | 10 ug | 200-R10n |
Umbilical Vein Endothelial Cell RNA (HUVEC RNA), Neonatal: Total RNA prepared from Human Umbilical Vein Endothelial Cells, neonatal | 25 ug | 200-R25n |
Human ICAM-1 ELISA Kit: Human Intercellular Adhesion Molecule-1 ELISA Kit | 96 Wells | CL0370 |
Human Gamma-Interferon Inducible Protein 10 (IP-10 / CXCL10): Human gamma-Interferon Inducible Protein 10 | 25 ug | RP1127-25 |
Human Gamma-Interferon Inducible Protein 10 (IP-10 / CXCL10): Human gamma-Interferon Inducible Protein 10 | 100 ug | RP1127-100 |
Human Gamma-Interferon Inducible Protein 10 (IP-10 / CXCL10): Human gamma-Interferon Inducible Protein 10 | 1000 ug | RP1127-1000 |
Human P-Selectin ELISA Kit: Human P-Selectin ELISA Kit | 96 wells | CL0505 |
Subculture Reagent Kit: 100 ml each of HBSS, Trypsin/EDTA & Trypsin Neutralizing Solution | 1 Kit | 090K |
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