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Human Umbilical Vein Smooth Muscle Cells: HUVSMC 人類臍靜脈平滑肌細胞：HUVSMC

Human Umbilical Vein Smooth Muscle Cells: HUVSMC 人類臍靜脈平滑肌細胞：HUVSMC

產品型號： 250K-05n

Human Umbilical Vein Smooth Muscle Cells (HUVSMC) from Cell Applications, Inc. have been used in studies evaluating treatments that involve blood vessel stents.

Specifically, intra-shunt delivery of motexafin gadolinium was shown to inhibit the proliferation of SMCs during treatment of portal hypertension

Also, high-density lipoproteins (HDL) exhibit cardioprotective and anti-inflammatory properties by reducing HUVSMC expression of chemokines CCL2, CCL5, and CX3CL1, and chemokine receptors CCR2 and CX3CR1 (via NF-κB/pAkt inhibition) and by decreasing cell proliferation (via pERK inhibition), highlighting HDL importance in preventing neointimal hyperplasia, the main cause of early vein graft/stent failure

HUVSMC were also used to demonstrate that genes coding for muscle and non-muscle actin proteins are transcribed by different transcription machinery

Characterization: positive for smooth muscle cell specific alpha-actin expression

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規格型錄

DETAILS

Tissue	Normal healthy human umbilical vein
QC	No bacteria, yeast, fungi, mycoplasma, virus
Character	Smooth muscle specific α-actin positive
Bioassay	Attach, spread, proliferate in Growth Med
Cryovial	500,000 HUVSMC (2nd passage) frozen in Basal Medium w/ 10% FBS, 10% DMSO
Kit	Cryovial frozen HUVSMC (250-05n), Growth Medium (311-500), Subcltr Rgnt Kit (090K)
Proliferating	Shipped in Gr Med, 3rd psg (flasks or plates)
Doublings	At least 6
Applications	Laboratory research use only (RUO). Not for human, clinical, diagnostic or veterinary use.

PRODUCTS

Product	Size	CAT.#
Cryopreserved Human Umbilical Vein Smooth Muscle Cell Total Kit, neonatal: 5x10^5 Cells (Adult), Medium & Subculture Reagents (See Details tab for specifics)	1 Kit	250K-05n
Cryopreserved Umbilical Vein Smooth Muscle Cells (HUVSMC), neonatal: Frozen HUVSMC (5x10^5)	1 Cryovial	250-05n

RELATED PRODUCTS

Product	Size	CAT.#
Human SMC Basal Medium: Basal medium (contains no growth supplement). Add GS before use.	500 ml	310-500
Human SMC Differentiation Medium: Promotes cells to change from one type to another, more specialized	250 ml	311D-250
Human SMC Growth Medium: All-in-one ready-to-use	500 ml	311-500
Human SMC Growth Medium Kit: Basal medium & growth supplement sold together packaged separately	Yields 500ml	311K-500
Human SMC Growth Supplement: Added to Basal Medium to create Growth Medium	30 ml	311-GS

EXTENDED FAMILY PRODUCTS

Product	Size	CAT.#
Mouse Interleukin-6 Antibody: Mouse Interleukin-6 Antibody	100 ul	CP10325
Freezing Medium: For general cryopreservation of most primary cells. Contains FBS & DMSO.	50 ml	040-50
Cytofect Smooth Muscle Cell Transfection Kit (125 x 24-Wells): 125 x 24-Well	1 Kit	TF350K
Cytofect Smooth Muscle Cell Transfection Sample Kit (25 x 24-Wells): 25 x 24-Well Rxns	1 Sample Kit	TF350KS
Umbilical Vein Smooth Muscle Cell RNA (HUVSMC RNA), Neonatal: Total RNA prepared from Human Umbilical Vein Smooth Muscle Cells, neonatal	10 ug	250-R10n
Umbilical Vein Smooth Muscle Cell RNA (HUVSMC RNA), Neonatal: Total RNA prepared from Human Umbilical Vein Smooth Muscle Cells, neonatal	25 ug	250-R25n
Human Interleukin-6 (IL-6): Human Interleukin-6	20 ug	RP1010-20
Human Interleukin-6 (IL-6): Human Interleukin-6	100 ug	RP1010-100
Human Interleukin-6 (IL-6): Human Interleukin-6	1000 ug	RP1010-1000
Human IL-6 ELISA Kit: Human Interleukin-6 ELISA Kit	96 Wells	CL0410
Subculture Reagent Kit: 100 ml each of HBSS, Trypsin/EDTA & Trypsin Neutralizing Solution	1 Kit	090K
Human IL-6, Animal Free: Human Interleukin-6, Animal-Free	20 ug	RP1010AF-20
Human IL-6, Animal Free: Human Interleukin-6, Animal-Free	100 ug	RP1010AF-100
Human IL-6, Animal Free: Human Interleukin-6, Animal-Free	1000 ug	RP1010AF-1000

General Instructions for Culturing Human Umbilical Vein Smooth Muscle Cells (HUVSMC)

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