Human Cardiac Fibroblasts: HCF 人類心臟纖維母細胞：HCF
產品型號： 306AK-05a
Human Cardiac Fibroblasts (HCF) are the most prevalent cell type in the heart. HCF from Cell Applications, Inc. provide an excellent model system to study many aspects of human heart function and pathophysiology.
HCF from Cell Applications, Inc. have been utilized in a numerous published studies, for example to:
- Determine that electrical coupling between cardiomyocytes and fibroblasts is mediated by Ca2+-activated K+ channels that can be stimulated by estrogen receptor agonists
- Show that antimitogenic effects of estradiol on HCF growth are mediated by cytochromes and metabolites
- Demonstrate that in response to mechanical stretch, cardiac fibroblasts release TGF-β that downregulates trombomodulin, increases thromboembolism and induces cardiac fibroblast differentiation into myofibroblasts
- Indicate that activation of G protein-coupled receptor kinase-2 prevents normal regulation of collagen synthesis in cardiac fibroblasts mimicking heart failure phenotype
- Identify FGF2 signaling pathway as potential target for modulating apoptosis in cardiac pathology
- Investigate the roles of scleraxis and AMPKα1 in scar formation following myocardial infarction
- Show that the KATP channel opener KMUP-3 preserved cardiac function after myocardial infarction by enhancing the expression of NO synthase and restoring MMP-9/TIMP-1 balance
DETAILS
|
Tissue
|
Normal healthy human heart tissue
|
|
QC
|
No bacteria, yeast, fungi, mycoplasma, virus
|
|
Bioassay
|
Attach, spread, proliferate in Growth Med
|
|
Cryovial
|
500,000 HCF (1st passage) frozen in Basal Medium w/ 10% FBS, 10% DMSO
|
|
Kit
|
Cryovial frozen HCF (306A-05a, adult atrium; 306V-05a, adult ventricle; or 306-05f fetal) , Growth Medium (316-500), Subcltr Rgnt Kit (090K)
|
|
Proliferating
|
Shipped in Gr Med, 2nd psg (flasks or plates)
|
|
Doublings
|
At least 8
|
|
Applications
|
Laboratory research use only (RUO). Not for human, clinical, diagnostic or veterinary use.
|
PRODUCTS
| Product | Size | CAT.# |
| Cryopreserved Human Cardiac Fibroblasts Total Kit, adult: 5x10^5 Cells (Adult), Medium & Subculture Reagents (See Details tab for specifics) | 1 Kit | 306AK-05a |
| Cryopreserved Cardiac Fibroblasts, Atrium, (HCF)adult: Frozen HCF (5x10^5) | 1 Cryovial | 306A-05a |
| Cryopreserved Human Cardiac Fibroblasts Total Kit, adult: 5x10^5 Cells (Adult), Medium & Subculture Reagents (See Details tab for specifics) | 1 Kit | 306VK-05a |
| Cryopreserved Cardiac Fibroblasts, Ventricle, (HCF), adult: Frozen HCF (5x10^5) | 1 Cryovial | 306V-05a |
| Cryopreserved Human Cardiac Fibroblasts Total Kit, adult: 5x10^5 Cells (Fetal), Medium & Subculture Reagents (See Details tab for specifics) | 1 Kit | 306K-05f |
| Cryopreserved Cardiac Fibroblasts (HCF), fetal: Frozen HCF (5x10^5) | 1 Cryovial | 306-05f |
RELATED PRODUCTS
| Product | Size | CAT.# |
| HCF Growth Medium: All-in-one ready-to-use | 500 ml | 316-500 |
| HCF Growth Medium Kit: Basal Medium and Growth Supplement sold together, bottled separately | yields 500 ml | 316K-500 |
| HCF Basal Medium: Basal medium (contains no growth supplement). Add GS before use. | 500 ml | 315-500 |
| HCF Growth Supplement: Added to Basal Medium to create Growth Medium | 50 ml | 316-GS |
EXTENDED FAMILY PRODUCTS
| Product | Size | CAT.# |
| Polyclonal Fibroblast Growth Factor 2 Rabbit Antibody: Polyclonal Fibroblast Growth Factor 2, FGF2 (bFGF), Rabbit Antibody | 100 ul | CA0259 |
| Polyclonal Fibroblast Growth Factor 7, KGF, Rabbit Antibody: Polyclonal Fibroblast Growth Factor 7, KGF, Rabbit Antibody | 100 ul | CA0853 |
| Rabbit Fibroblast Growth Factor 8 Antibody: Rabbit Fibroblast Growth Factor 8 Antibody | 100 ul | CA1216 |
| Mouse Fibroblast Growth Factor Receptor 1 Antibody: Mouse Fibroblast Growth Factor Receptor 1 Antibody | 100 ul | CP10102 |
| Polyclonal Fibroblast Growth Factor-Receptor 2 Antibody: Polyclonal Fibroblast Growth Factor-Receptor 2 Antibody | 100 ul | CB4365 |
| Mouse Fibroblast Growth Factor Receptor Tyrosine Kinase 4 Antibody: Mouse Fibroblast Growth Factor Receptor Tyrosine Kinase 4 Antibody | 100 ul | CP10103 |
| Rabbit Macrophage Migration Inhibitory Factor Antibody: Rabbit Macrophage Migration Inhibitory Factor Antibody | 100 ul | CA1052 |
| Cytofect Fibroblast Transfection Kit (250 x 24-Wells): 250 x 24-Well Rxns | 1 Kit | TF103K |
| Cytofect Fibroblast Transfection Sample Kit (25 x 24-Wells): 25 x 24-Well Rxns | 1 Sample Kit | TF103KS |
| Human Fibroblast Growth Factor-21 (FGF-21): Human Fibroblast Growth Factor-21 | 20 ug | RP1148-20 |
| Human Fibroblast Growth Factor-21 (FGF-21): Human Fibroblast Growth Factor-21 | 100 ug | RP1148-100 |
| Human Fibroblast Growth Factor-21 (FGF-21): Human Fibroblast Growth Factor-21 | 1000 ug | RP1148-1000 |
| Human Fibroblast Growth Factor-22 (FGF-22): Human Fibroblast Growth Factor-22 | 20 ug | RP1068-20 |
| Human Fibroblast Growth Factor-22 (FGF-22): Human Fibroblast Growth Factor-22 | 100 ug | RP1068-100 |
| Human Fibroblast Growth Factor-22 (FGF-22): Human Fibroblast Growth Factor-22 | 1000 ug | RP1068-1000 |
| Human Fibroblast Growth Factor Acidic (FGF-1): Human Fibroblast Growth Factor-acidic | 10 ug | RP1029-10 |
| Human Fibroblast Growth Factor Acidic (FGF-1): Human Fibroblast Growth Factor-acidic | 50 ug | RP1029-50 |
| Human Fibroblast Growth Factor Acidic (FGF-1): Human Fibroblast Growth Factor-acidic | 1000 ug | RP1029-1000 |
| Human FGF9 ELISA Kit: Human Fibroblast Growth Factor 9 ELISA Kit | 96 Wells | CL0348 |
| Cardiac Fibroblast RNA (HCF RNA), Fetal: Total RNA prepared from Human Cardiac Fibroblasts, fetal | 10 ug | 306-R10f |
| Cardiac Fibroblast RNA (HCF RNA), Fetal: Total RNA prepared from Human Cardiac Fibroblasts, fetal | 25 ug | 306-R25f |
| Cardiac Fibroblast RNA (HCF RNA), Adult: Total RNA prepared from Human Cardiac Fibroblasts, adult | 10 ug | 306-R10a |
| Cardiac Fibroblast RNA (HCF RNA), Adult: Total RNA prepared from Human Cardiac Fibroblasts, adult | 25 ug | 306-R25a |
| Human Heart RNA: Total RNA prepared from human heart tissue | 50 ug | 1H30-50 |
| Human Heart RNA: Total RNA prepared from human heart tissue | 250 ug | 1H30-250 |
| Subculture Reagent Kit: 100 ml each of HBSS, Trypsin/EDTA & Trypsin Neutralizing Solution | 1 Kit | 090K |
| Human Fibroblast Growth Factor-4 (FGF-4): Human Fibroblast Growth Factor-4 | 25 ug | RP1031-25 |
| Human Fibroblast Growth Factor-4 (FGF-4): Human Fibroblast Growth Factor-4 | 100 ug | RP1031-100 |
| Human Fibroblast Growth Factor-4 (FGF-4): Human Fibroblast Growth Factor-4 | 1000 ug | RP1031-1000 |
| Human Fibroblast Growth Factor 147 Basic (FGF-147 basic/FGF-2): Human Fibroblast Growth Factor-147-basic | 10 ug | RP1028-10 |
| Human Fibroblast Growth Factor 147 Basic (FGF-147 basic/FGF-2): Human Fibroblast Growth Factor-147-basic | 100 ug | RP1028-100 |
| Human Fibroblast Growth Factor 147 Basic (FGF-147 basic/FGF-2): Human Fibroblast Growth Factor-147-basic | 1000 ug | RP1028-1000 |
| Human Fibroblast Growth Factor 154 Basic (FGF-basic 154 / FGF-2): Human Fibroblast Growth Factor-154-basic | 10 ug | RP1146-10 |
| Human Fibroblast Growth Factor 154 Basic (FGF-basic 154 / FGF-2): Human Fibroblast Growth Factor-154-basic | 100 ug | RP1146-100 |
| Human Fibroblast Growth Factor 154 Basic (FGF-basic 154 / FGF-2): Human Fibroblast Growth Factor-154-basic | 1000 ug | RP1146-1000 |
| Human Granulocyte Macrophage Colony Stimulating Factor (GM-CSF): Human Granulocyte Macrophage-Colony Stimulating Factor | 20 ug | RP1008-20 |
| Human Granulocyte Macrophage Colony Stimulating Factor (GM-CSF): Human Granulocyte Macrophage-Colony Stimulating Factor | 100 ug | RP1008-100 |
| Human Granulocyte Macrophage Colony Stimulating Factor (GM-CSF): Human Granulocyte Macrophage-Colony Stimulating Factor | 1000 ug | RP1008-1000 |
| Human Gamma-Interferon Inducible Protein 10 (IP-10 / CXCL10): Human gamma-Interferon Inducible Protein 10 | 25 ug | RP1127-25 |
| Human Gamma-Interferon Inducible Protein 10 (IP-10 / CXCL10): Human gamma-Interferon Inducible Protein 10 | 100 ug | RP1127-100 |
| Human Gamma-Interferon Inducible Protein 10 (IP-10 / CXCL10): Human gamma-Interferon Inducible Protein 10 | 1000 ug | RP1127-1000 |
| Human Migration Inhibitory Factor (MIF): Human Migration Inhibitory Factor | 25 ug | RP1153-25 |
| Human Migration Inhibitory Factor (MIF): Human Migration Inhibitory Factor | 100 ug | RP1153-100 |
| Human Migration Inhibitory Factor (MIF): Human Migration Inhibitory Factor | 1000 ug | RP1153-1000 |
| Human MIF ELISA Kit: Human Macrophage Migration Inhibitory Factor ELISA Kit | 96 wells | CL0813 |
| Human GM-CSF, Animal-Free: Human Granulocyte Macrophage-Colony Stimulating Factor, Animal-Free | 20 ug | RP1008AF-20 |
| Human GM-CSF, Animal-Free: Human Granulocyte Macrophage-Colony Stimulating Factor, Animal-Free | 100 ug | RP1008AF-100 |
| Human GM-CSF, Animal-Free: Human Granulocyte Macrophage-Colony Stimulating Factor, Animal-Free | 1000 ug | RP1008AF-1000 |
| Human FGF-147 basic, Animal-Free: Human Fibroblast Growth Factor-basic-147, Animal-Free | 10 ug | RP1028AF-10 |
| Human FGF-147 basic, Animal-Free: Human Fibroblast Growth Factor-basic-147, Animal-Free | 100 ug | RP1028AF-100 |
| Human FGF-147 basic, Animal-Free: Human Fibroblast Growth Factor-basic-147, Animal-Free | 1000 ug | RP1028AF-1000 |
| Human FGF-basic 154, Animal-Free: Human Fibroblast Growth Factor-basic-154, Animal-Free | 10 ug | RP1146AF-10 |
| Human FGF-basic 154, Animal-Free: Human Fibroblast Growth Factor-basic-154, Animal-Free | 100 ug | RP1146AF-100 |
| Human FGF-basic 154, Animal-Free: Human Fibroblast Growth Factor-basic-154, Animal-Free | 1000 ug | RP1146AF-1000 |
Inline content