Human Coronary Artery Smooth Muscle Cells: HCASMC 人類冠狀動脈平滑肌細胞：HCASMC
產品型號： 350ASK-05a
Human Coronary Artery Smooth Muscle Cells (HCASMC) provide an excellent model system to study all aspects of cardiovascular function and disease, especially those related to mechanisms of hyperplasia and hypertrophy of intimal smooth muscle cells leading to vascular occlusion in atherosclerosis and stent restenosis.
HCASMC from Cell Applications, Inc. have been utilized in a number of research studies, for example, to:
- Study signaling pathways regulating smooth muscle differentiation and chronic inflammation of arterial wall that leads to artherosclerosis
- Demonstrate that STAT-1 and STAT-3 regulate VEGF production in smooth muscle cells by having opposing effects on HIF-1α expression
- Examine the mechanisms of hypoxia and reoxigenation injuries in by demonstrating increased production of ROS and inflammatory cytokines, and further showing that DHA is not beneficial in this type of injuries
- Investigate (by also using human Internal Thoracic Artery Smooth Muscle Cells obtained from Cell Applications, Inc.), the gene expression differences between smooth muscle cells from different arteries, underlying their differential response to injuries and proliferation stimuli
- Suggest the hypermethylation of SOCS3 gene as the connection between TNF-α and IGF-1 released in response to mechanical injury during coronary intervention, and the induction of cytokines leading to intimal hyperplasia and restenosis
- Develop a novel VEGFR/MET-targeted inhibitor with improved antitumor efficacy and decreased toxicity
- Investigate novel therapies and drug combinations to achieve optimal target selectivity
- Generate elastic scaffolds for tissue engineering and novel treatment strategies to prevent stent restenosis by designing new materials, or drug therapies to preferentially inhibit smooth muscle cell growth
Characterization: positive for smooth muscle cell specific alpha-actin expression
DETAILS
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Tissue
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Normal healthy human coronary artery
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QC
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No bacteria, yeast, fungi, mycoplasma, virus
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Character
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Smooth muscle specific α-actin positive
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Bioassay
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Attach, spread, proliferate in Growth Med
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Cryovial
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500,000 HCASMC (2nd passage) frozen in Basal Medium w/ 10% FBS, 10% DMSO
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Kit
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Cryovial frozen HCASMC (350-05a), Growth Medium (311-500), Subcltr Rgnt Kit (090K)
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Proliferating
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Shipped in Gr Med, 3rd psg (flasks or plates)
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Doublings
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At least 16
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Applications
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Laboratory research use only (RUO). Not for human, clinical, diagnostic or veterinary use.
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PRODUCTS
| Product | Size | CAT.# |
| Cryopreserved Human Coronary Artery Smooth Muscle Cells Total Kit: 5x10^5 Cells (Adult), Medium & Subculture Reagents (See Details tab for specifics) | 1 Kit | 350K-05a |
| Cryopreserved Coronary Artery Smooth Muscle Cells (HCASMC), adult: Frozen HCASMC (5x10^5) | 1 Cryovial | 350-05a |
| Cryopreserved Human Coronary Artery Smooth Muscle Cells Total Kit, adult, pooled: 5x10^5 Cells (Pooled, Adult), Medium & Subculture Reagents (See Details tab for specifics) | 1 Kit | 350pK-05a |
| Cryopreserved Coronary Artery Smooth Muscle Cells (P-HCASMC) Pooled, adult: Frozen HCASMC (5x10^5) | 1 Cryovial | 350p-05a |
| Cryopreserved Human Coronary Artery Smooth Muscle Cells with plaque Total Kit, adult: 5x10^5 Cells (Plaque, Adult), Medium & Subculture Reagents (See Details tab for specifics) | 1 Kit | 350qK-05a |
| Cryopreserved Coronary Artery Smooth Muscle Cells (HCASMC) Plaque, adult: Frozen HCASMC (5x10^5) | 1 Cryovial | 350q-05a |
| Cryopreserved Human Coronary Artery Smooth Muscle Cells with Asthma Total Kit: 5x10^5 Cells (from donor with Asthma, Adult), Medium & Subculture Reagents (See Details tab for specifics) | 1 Kit | 350ASK-05a |
| Cryopreserved Coronary Artery Smooth Muscle Cells, Asthma (HCASMC-AS), adult: Frozen HCASMC-AS from donor with Asthma (5x10^5) | 1 Cryovial | 350AS-05a |
RELATED PRODUCTS
| Product | Size | CAT.# |
| Human SMC Growth Medium: All-in-one ready-to-use | 500 ml | 311-500 |
| Human SMC Growth Medium Kit: Basal medium & growth supplement sold together packaged separately | Yields 500ml | 311K-500 |
| Human SMC Basal Medium: Basal medium (contains no growth supplement). Add GS before use. | 500 ml | 310-500 |
| Human SMC Growth Supplement: Added to Basal Medium to create Growth Medium | 30 ml | 311-GS |
| Human SMC Differentiation Medium: Promotes cells to change from one type to another, more specialized | 250 ml | 311D-250 |
| HCASMC Conditioned Medium: HCASMC Conditioned Medium | 5 ml | 350cm-005 |
EXTENDED FAMILY PRODUCTS
| Product | Size | CAT.# |
| Mouse Interleukin-6 Antibody: Mouse Interleukin-6 Antibody | 100 ul | CP10325 |
| Cytofect Smooth Muscle Cell Transfection Kit (125 x 24-Wells): 125 x 24-Well | 1 Kit | TF350K |
| Cytofect Smooth Muscle Cell Transfection Sample Kit (25 x 24-Wells): 25 x 24-Well Rxns | 1 Sample Kit | TF350KS |
| Coronary Artery Smooth Muscle Cell RNA (HCASMC RNA), Adult: Total RNA prepared from Human Coronary Artery Smooth Muscle Cells, adult | 10 ug | 350-R10a |
| Coronary Artery Smooth Muscle Cell RNA (HCASMC RNA), Adult: Total RNA prepared from Human Coronary Artery Smooth Muscle Cells, adult | 25 ug | 350-R25a |
| Human Heart RNA: Total RNA prepared from human heart tissue | 50 ug | 1H30-50 |
| Human Heart RNA: Total RNA prepared from human heart tissue | 250 ug | 1H30-250 |
| Human Interleukin-6 (IL-6): Human Interleukin-6 | 20 ug | RP1010-20 |
| Human Interleukin-6 (IL-6): Human Interleukin-6 | 100 ug | RP1010-100 |
| Human Interleukin-6 (IL-6): Human Interleukin-6 | 1000 ug | RP1010-1000 |
| Human IL-6 ELISA Kit: Human Interleukin-6 ELISA Kit | 96 Wells | CL0410 |
| Subculture Reagent Kit: 100 ml each of HBSS, Trypsin/EDTA & Trypsin Neutralizing Solution | 1 Kit | 090K |
| Human IL-6, Animal Free: Human Interleukin-6, Animal-Free | 20 ug | RP1010AF-20 |
| Human IL-6, Animal Free: Human Interleukin-6, Animal-Free | 100 ug | RP1010AF-100 |
| Human IL-6, Animal Free: Human Interleukin-6, Animal-Free | 1000 ug | RP1010AF-1000 |
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