Rat Cardiomyocytes: RCm 大鼠心肌細胞：RCm
產品型號： R357-25-3
Rat Cardiomyocytes (RCm) are primary cells derived from normal rat heart tissue and plated on inserts or plates immediately after primary isolation. RCm retain their physiological functions (including beating) and provide a useful in vitro model to study cardiotoxicity.
RCm from Cell Applications, Inc. have been used to investigate mechanisms of mitochondrial and cardiovascular toxicity exhibited by anti-cancer drugs targeting angiogenesis. Antipsychotic drugs, thioridazine and mesoridazine, were shown to inhibit gap junction communication and affect the beating of RCm, explaining the cardiac side-effects observed in some patients. Clostridium difficile toxin B was shown to disrupt coordinated contractility and rhythmicity in RCm, but the damage was prevented by a caspase-3 inhibitor.
Additionally, RCm were used to demonstrate that Mas receptor, a GPCR activates phospholipase C and causes inositol phosphate accumulation, contributing to cardiac ischemia-reperfusion injury. The same system was then used to screen for agonists and inverse agonists for Mas. Inhibiting Mas function provided cardioprotective effects in mice and in isolated rat hearts. RCm were also used to develop a new probe for positron emission tomography that allows for more effective cardiac imaging.
DETAILS
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Tissue
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Normal healthy neonatal rat heart
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QC
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No bacteria, yeast, fungi, mycoplasma |
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Bioassay
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Attaches on FBS-coated culture ware, Remains viable 2-4 days in Cltr Med |
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Cryovial
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N/A |
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Kit
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N/A |
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Cultured
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Shipped w/in 3 days after plating in Maint Med (flasks or plates)
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Doublings
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N/A |
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Applications
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Laboratory research use only (RUO). Not for human, clinical, diagnostic or veterinary use. |
PRODUCTS
| PRODUCT | SIZE | CAT.# |
| Cultured RCm: Fresh, Plated Cells in Maintenance Medium | 3 of T-25 Flasks | R357-25-3 |
| Cultured RCm: Fresh, Plated Cells in Maintenance Medium | 2 of T-75 Flask | R357-75-2 |
RELATED PRODUCTS
| PRODUCT | SIZE | CAT.# |
| RCm Culture Medium: All-in-one ready-to-use media | 500 ml | R313-500 |
| RCm Culture Medium Kit: Basal medium & growth supplement sold together packaged separately | Yields 500 ml | R313K-500 |
| RCm Basal Medium: Basal medium (contains no growth supplement). Add GS before use. | 500 ml | R312-500 |
| RCm Culture Supplement: Added to Basal Medium to create Culture Medium | 80 ml | R313-GS |
| RCm Culture Medium wo FBS: Culture medium without FBS | 500 ml | R313F-500 |
| RCm Culture Medium w/ FBS or Horse Serum: Culture medium without FBS and horse serum | 500 ml | R313FH-500 |
EXTENDED FAMILY PRODUCTS
| PRODUCT | SIZE | CAT.# |
| Freezing Medium: For general cryopreservation of most primary cells. Contains FBS & DMSO. | 50 ml | 040-50 |
Inline content