Human Skeletal Muscle Cells: HSkMC: Pre-Screened 人體骨骼肌細胞：HSkMC：預篩選
產品型號： S150K-05f
Pre-Screened for Insulin & AMPK signaling are isolated from the skeletal muscle of hamstrings and retain morphological, biochemical, and metabolic characteristics of skeletal muscle. Pre-screened HSkMCs can undergo differentiation to exhibit actin and myosin myofilaments and are specially tested for functional AMPK & Insulin Signaling Pathways.
AMPK Signaling Pathway
AMP-activated protein kinase (AMPK) is a major cellular regulator of lipid and glucose metabolism and mediates the metabolic changes associated with exercise. AMPK phosphorylates and inhibits activity of acetyl CoA carboxylase (ACC), the enzyme responsible for making malonyl-CoA which is required for fatty acid chain elongation.
Insulin Signaling Pathway
Skeletal muscle is one of major target tissue of insulin action. Upon insulin binding, insulin receptor tyrosine kinases catalyze autophosphorylation of tyrosine residues providing docking sites for downstream signaling components, such as IRS-1 and Grb2, which relay the signaling further into the cell (see Fig.B).
(Click to Enlarge) Human Skeletal Muscle Cells (HSkMC) response to forced activation of AMPK, leading to ACC phosphorylation. Cells serum-starved 16 hr & treated for 0, 15 or 30 min w/ 100nM oligomycin, which inhibits oxidative phosphorylation & causes activation of AMPK (A). Simplified insulin signal transduction map. Specific phosphorylation events examined in Fig. C are marked in red (B). HSkMC response to insulin signaling leading to phosphorylation of IRS-1, Akt, GSK-3b, MAPK/ERK and p70S6K. Cells serum-starved 16 hr & stimulated w/ 100nM insulin for 0, 15 or 30 min.
DETAILS
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Tissue
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Normal healthy human limb skeletal muscle
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QC
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No bacteria, yeast, fungi, mycoplasma, virus
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Cryovial
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500,000 HSkMC (2nd passage) frozen in Basal Medium w/ 10% FBS, 10% DMSO
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Kit
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Cryovial frozen HSkMC (150-05f), Growth Medium (151-500), Subcltr Rgnt Kit (090K)
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Proliferating
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Shipped in Gr Med, 3rd psg (flasks or plates)
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Doublings
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At least 15
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Applications
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Laboratory research use only (RUO). Not for human, clinical, diagnostic or veterinary use.
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PRODUCTS
| PRODUCT | SIZE | CAT.# |
| Cryopreserved HSkMC, Pre-Screened, Fetal: 5x10^5 Cells (Pre-Screened, Fetal), Medium & Subculture Reagents (See Details tab for specifics) | 1 Kit | S150K-05f |
| Cryopreserved HSkMC, Pre-Screened, Fetal: Frozen HSkMC (5x10^5) | 1 Cryovial | S150-05f |
| Cryopreserved HSkMC-COPD, Pre-Scr, Adult: Frozen HSkMC-COPD from donor with Chronic Obstructive Pulmonary Disease, Adult (5x10^5) | 1 Cryovial | S150COPD-05a |
| Cryopreserved HSkMC-COPD, Pre-Scr, Adult: 5x10^5 Cells (from donor with COPD, Pre-Screened, Adult), Medium & Subculture Reagents (See Details tab for specifics) | 1 Kit | S150COPDK-05a |
| Proliferating HSkMC, Pre-Screened, Fetal: Actively growing, dividing cells in medium | T-25 Flask | S151-25f |
| Proliferating HSkMC, Pre-Screened, Fetal: Actively growing, dividing cells in medium | T-75 Flask | S151-75f |
RELATED PRODUCTS
| PRODUCT | SIZE | CAT.# |
| HSkMC Basal Medium: Basal medium (contains no growth supplement). Add GS before use. | 500 ml | 150-500 |
| HSkMC Differentiation Medium: Promotes cells to change from one type to another, more specialized | 250 ml | 151D-250 |
| HSkMC Growth Medium: All-in-one ready-to-use | 500 ml | 151-500 |
| HSkMC Growth Medium Kit: Basal medium & growth supplement sold together packaged separately | Yields 500 ml | 151K-500 |
| HSkMC Growth Supplement: Added to Basal Medium to create Growth Medium | 50 ml (2 parts) | 151-GS |
| Proliferating HSkMC, Pre-Screened, Adult : Actively growing, dividing cells in medium | T-75 Flask | S151-75a |
EXTENDED FAMILY PRODUCTS
| PRODUCT | SIZE | CAT.# |
| Mouse Interleukin-6 Antibody: Mouse Interleukin-6 Antibody | 100 ul | CP10325 |
| Mouse Myostatin Antibody: Mouse Myostatin Antibody | 100 ul | CP10315 |
| Freezing Medium: For general cryopreservation of most primary cells. Contains FBS & DMSO. | 50 ml | 040-50 |
| HSkMC RNA, Fetal: Total RNA prepared from Human Skeletal Muscle Cells, fetal | 10 ug | 150-R10f |
| HSkMC RNA, Fetal: Total RNA prepared from Human Skeletal Muscle Cells, fetal | 25 ug | 150-R25f |
| Human Interleukin-6 (IL-6): Human Interleukin-6 | 20 ug | RP1010-20 |
| Human Interleukin-6 (IL-6): Human Interleukin-6 | 100 ug | RP1010-100 |
| Human Interleukin-6 (IL-6): Human Interleukin-6 | 1000 ug | RP1010-1000 |
| Human Myostatin (GDF-8): Human Myostatin | 10 ug | RP1022-10 |
| Human Myostatin (GDF-8): Human Myostatin | 100 ug | RP1022-100 |
| Human Myostatin (GDF-8): Human Myostatin | 1000 ug | RP1022-1000 |
| Human Myostatin Propeptide: Human Myostatin Propeptide | 25 ug | RP1142-25 |
| Human Myostatin Propeptide: Human Myostatin Propeptide | 100 ug | RP1142-100 |
| Human Myostatin Propeptide: Human Myostatin Propeptide | 1000 ug | RP1142-1000 |
| Human Skeletal Muscle RNA: Total RNA prepared from human skeletal muscle tissue | 50 ug | 1H60-50 |
| Human Skeletal Muscle RNA: Total RNA prepared from human skeletal muscle tissue | 250 ug | 1H60-250 |
| Subculture Reagent Kit: 100 ml each of HBSS, Trypsin/EDTA & Trypsin Neutralizing Solution | 1 Kit | 090K |
| Human IL-6, Animal Free: Human Interleukin-6, Animal-Free | 20 ug | RP1010AF-20 |
| Human IL-6, Animal Free: Human Interleukin-6, Animal-Free | 100 ug | RP1010AF-100 |
| Human IL-6, Animal Free: Human Interleukin-6, Animal-Free | 1000 ug | RP1010AF-1000 |
| Skeletal Muscle Cell Starvation Medium: Use when cells need to be starved overnight to 24 hrs before experiment | 250 ml | 151S-250 |
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